Hwang Sun Young, Park Young Kyung, Koo Hye Cheong, Park Yong Ho
Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2010 Jun;11(2):125-31. doi: 10.4142/jvs.2010.11.2.125.
Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I nuSabeta. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently.
金黄色葡萄球菌是牛乳腺炎的主要病原，在全球奶牛群中引发重大经济损失。本研究通过spa分型和葡萄球菌肠毒素（SE）基因分析对从韩国患乳腺炎奶牛乳汁中分离出的金黄色葡萄球菌菌株进行了调查。从五个省份的26个农场分离出44株金黄色葡萄球菌菌株。基于14种spa型，所有分离株分为五个簇和两个单株。簇1和簇2分离株分别占总分离株的38.6%和36.4%，分布在四个以上省份。在34株（77.3%）分离株中检测到SE和SE样毒素基因，最常检测到的SE基因谱是seg、sei、selm、seln和selo基因（16株，36.3%），这与基因组岛之一I型nuSabeta相当。这是韩国牛生鲜乳中金黄色葡萄球菌分离株的spa型以及最近描述的SE和SE样毒素基因流行情况的首次报告。两个主要的spa组分布广泛，最近描述的SE和SE样毒素基因检测频繁。
