Petróczki Flóra M, Pásztor Ákos, Szűcs Kata D, Pál Károly, Kardos Gábor, Albert Ervin, Horváth Brigitta, Ungvári Erika, Béri Béla, Peles Ferenc
Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Science, University of Debrecen, 4032 Debrecen, Hungary.
Faculty of Agricultural and Food Sciences and Environmental Management, Doctoral School of Animal Science, University of Debrecen, 4032 Debrecen, Hungary.
Pathogens. 2021 Jan 21;10(2):104. doi: 10.3390/pathogens10020104.
In this research, our aim was to assess the occurrence of in a Hungarian large-scale dairy farm during the control program conducted in the course of our studies. Furthermore, the phenotypic and genotypic properties of the isolates (type of haemolysis, antibiotic susceptibility, staphylococcal enterotoxin (SE) gene carrying ability and type) were determined. was detected in all bulk tank milk samples collected during this study. Two different types were identified among the 17 strains isolated in the farm. A total of 14 of the 17 studied strains (82%) showed β-haemolysis on blood agar, 2/17 strains (12%) expressed double zone and 1/17 strains (6%) showed weak β-haemolysis. All strains were susceptible to most antibiotics tested (cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, tetracycline and trimethoprim/sulphamethoxazole), but all strains were resistant to penicillin G. A total of 11 of the 17 strains (65%) were found to harbour , , , , genes; 4/17 strains (24%) harboured , , , genes and 2/17 strains (11%) harboured gene. Since the new SEs/SEls can also cause foodborne outbreaks potentially and all strains were found to be resistant to penicillin G, it is essential to decrease and keep the prevalence of low in the dairy farm and the implementation of the control program is also highly justified. The results showed that the count decreased by the end of our studies, so the control program was proved to be effective.
在本研究中，我们的目的是评估在我们研究过程中实施的控制计划期间，匈牙利一个大型奶牛场中[具体细菌名称未给出]的发生情况。此外，还确定了分离株的表型和基因型特性（溶血类型、抗生素敏感性、携带葡萄球菌肠毒素（SE）基因的能力以及[具体细菌类型未给出]类型）。在本研究期间采集的所有散装罐牛奶样本中均检测到了[具体细菌名称未给出]。在该农场分离出的17株菌株中鉴定出两种不同的[具体细菌类型未给出]类型。在17株研究菌株中，共有14株（82%）在血平板上显示β溶血，2/17株（12%）表现为双区溶血，1/17株（6%）显示弱β溶血。所有菌株对大多数测试抗生素（头孢西丁、氯霉素、克林霉素、红霉素、庆大霉素、四环素和甲氧苄啶/磺胺甲恶唑）敏感，但所有菌株对青霉素G耐药。在17株菌株中，共有11株（65%）被发现携带[具体基因未给出]、[具体基因未给出]、[具体基因未给出]、[具体基因未给出]、[具体基因未给出]基因；4/17株（24%）携带[具体基因未给出]、[具体基因未给出]、[具体基因未给出]、[具体基因未给出]基因，2/17株（11%）携带[具体基因未给出]基因。由于新的SEs/SEls也可能引发食源性疾病暴发，且所有菌株均对青霉素G耐药，因此降低并保持奶牛场中[具体细菌名称未给出]的流行率至关重要，实施[具体控制计划名称未给出]控制计划也非常合理。结果表明，在我们的研究结束时，[具体细菌名称未给出]数量有所下降，因此证明控制计划是有效的。
