Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Arch Virol. 2010 Jul;155(7):1157-61. doi: 10.1007/s00705-010-0679-z. Epub 2010 May 11.
By reverse transcription (RT)-nested polymerase chain reaction (PCR), hepatitis E virus (HEV) RNA was simultaneously detected in the livers of aborted fetuses and in fecal and serum samples from their sows, obtained from two of 14 farms tested. Sequence and phylogenetic analyses showed that these HEVs belonged to genotype 3 HEV. HEV RNA was detected by real-time RT-PCR at 2.0 x 10(3)-2.8 x 10(4) viral copies/microl in the HEV-positive samples. All HEV-positive fetuses tested positive for porcine circovirus type 2 (PCV2). These results indicate that transplacental infection of HEV with PCV2 coinfection may occur in sows with reproductive failure, which is suggestive of similarities to HEV infection in women.
通过反转录（RT）-巢式聚合酶链反应（PCR），在来自两个受检的 14 个农场的流产胎儿肝脏以及其母猪的粪便和血清样本中同时检测到戊型肝炎病毒（HEV）RNA。序列和系统发生分析表明，这些 HEV 属于基因型 3 HEV。通过实时 RT-PCR 检测，HEV 阳性样本中的 HEV RNA 为 2.0 x 10(3)-2.8 x 10(4) 病毒拷贝/微升。所有检测到的 HEV 阳性胎儿均对猪圆环病毒 2 型（PCV2）呈阳性。这些结果表明，在繁殖失败的母猪中可能发生伴有 PCV2 共感染的 HEV 胎盘感染，这表明与女性 HEV 感染有相似之处。
