Xu Jin-sen, Pan Xiao-hua, Hu Xiang-long, Sa Zhe-yan, Zheng Shu-xia
Department of Meridian Research, Fu-jian Institute of Chinese Medicine, Fuzhou 350003, China.
Zhen Ci Yan Jiu. 2010 Feb;35(1):47-51.
To observe the effect of electroacupuncture (EA) of different acupoints on electroretinogram (ERG) and cerebral visual evoked potentials (VEP) in healthy subjects so as to evaluate the relative specificity of the function of acupoints.
A total of 33 healthy adult volunteers were observed in the present study. The subject was asked to lie on a testing bed for a while, two disk electrodes were respectively fixed to the suborbital region and the occiput (the crossing point, 5 cm apart from the right median line of the head and 5 cm above the ear), and the reference electrode was fixed to the medial side of the earlobe for recording ERG and cerebral VEP separately. The testing room was asked to keep faint light during the whole recording process. A flash stimulation (frequency 1 Hz) was applied to the subject's eye (with a distance of about 50 cm between the light source and the tested eye, and the other eye was covered by an eyepatch). EA (0.5 Hz, 0.2 ms and 3-4 V) was applied to Guangming (GB 37), Neiguan (PC 6) and Zusanli (ST 36) for 30 min respectively in different testing days (with the interval being 3 days at least). ERG and cerebral VEP were recorded by using Polygraphy (RM-6200).
Following EA of Guangming (GB 37), Neiguan (PC 6) and Zusanli (ST 36),the changing values (increase and decrease) of the amplitude of b waves of ERG and P100 waves of cerebral VEP were (19.68 +/- 12.61)% and (12.84 +/- 14.08)%, (6.09 +/- 14.06)% and (5.61 +/- 8.01)%, (2.39 +/- 3.52)% and (5.43 +/- 8.22)%, respectively, while those of b waves of ERG and P100 waves of cerebral VEP of GB 37 were significantly bigger than those of PC 6 and ST 36 (P < 0.01).
EA of Guangming (GB 37)can significantly change the amplitude of both ERG and cerebral VEP in volunteer subjects, which is markedly bigger than those of EA of Zusanli (ST 36) and Neiguan (PC 6), suggesting a relative specificity of the effect of EA of different acupoints.
观察针刺不同穴位对健康受试者视网膜电图（ERG）及大脑视觉诱发电位（VEP）的影响，以评价穴位功能的相对特异性。
本研究共观察33名健康成年志愿者。受试者先在检查床上躺片刻，将两个圆盘电极分别固定于眶下区和枕部（枕部为头部右正中线旁开5 cm、耳上5 cm处的交叉点），参考电极固定于耳垂内侧，分别记录ERG和大脑VEP。整个记录过程中，检查室保持昏暗灯光。对受试者一眼进行闪光刺激（频率1 Hz）（光源与受试眼距离约50 cm，另一眼用眼罩遮盖）。在不同检查日（间隔至少3天）分别对光明（GB 37）、内关（PC 6）和足三里（ST 36）进行电针刺激（频率0.5 Hz，波宽0.2 ms，强度3 - 4 V），持续30分钟。采用多导生理记录仪（RM - 6200）记录ERG和大脑VEP。
针刺光明（GB 37）、内关（PC 6）和足三里（ST 36）后，ERG的b波振幅及大脑VEP的P100波振幅的变化值（升高和降低）分别为（19.68±12.61）%和（12.84±14.08）%、（6.09±14.06）%和（5.61±8.01）%、（2.39±3.52）%和（5.43±8.22）%，而GB 37的ERG的b波振幅及大脑VEP的P100波振幅变化值明显大于PC 6和ST 36（P < 0.01）。
针刺光明（GB 37）能显著改变志愿者ERG及大脑VEP的振幅，且明显大于针刺足三里（ST 36）和内关（PC 6），提示不同穴位电针效应具有相对特异性。
