Li Chunying, Zhang Xiaojun
Graduate School of Northeast Forestry University, Harbin 150040, China.
Se Pu. 2010 Jan;28(1):64-7. doi: 10.3724/sp.j.1123.2010.00064.
A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lobetyolin, pachymic acid, glycyrrhizic acid, atractylenoide III and atractylenolide I in Sijunzi bolus. The separation was performed on an HIQ SIL C18 V column (250 mm x 4.6 mm, 5 microm) with 0.5% acetic acid-methanol as the mobile phase of gradient elution at a flow rate of 1.0 mL/min. The detection was performed with an evaporation light scattering detector (ELSD) and the sample volume was 10 microL. The temperature of drift tube and heating grade of nebulizer was respectively set at 55 degrees C and 60% at 0.2 MPa of pressure. Nitrogen gas was used as carrier gas. Under the optimized conditions, there were good linear relationships between the logarithm values of mass concentration and the peak areas of lobetyolin, pachymic acid, glycyrrhizic acid, atractylenoide III and atractylenolide I in the ranges of 0.076 - 1.21, 0.048 -0.76, 0.153 - 2.45, 0.045 - 0.72 and 0.098 - 1.56 g/L, respectively. The recoveries of the five components were between 97.13% and 100.25%, the relative standard deviations (RSDs) were between 1.23% and 2.44%. This method is simple, rapid, accurate and suitable for the quality control of Sijunzi bolus.
建立了一种高效液相色谱（HPLC）法，用于同时测定四君子丸中党参炔苷、茯苓酸、甘草酸、苍术烯内酯III和苍术内酯I的含量。采用HIQ SIL C18 V柱（250 mm×4.6 mm，5μm）进行分离，以0.5%乙酸-甲醇为流动相进行梯度洗脱，流速为1.0 mL/min。采用蒸发光散射检测器（ELSD）进行检测，进样量为10μL。漂移管温度和雾化器加热梯度分别设定为55℃和60%，压力为0.2 MPa。以氮气作为载气。在优化条件下，党参炔苷、茯苓酸、甘草酸、苍术烯内酯III和苍术内酯I的质量浓度对数与峰面积在0.076 - 1.21、0.048 - 0.76、0.153 - 2.45、0.045 - 0.72和0.098 - 1.56 g/L范围内均具有良好的线性关系。5种成分的回收率在97.13%至100.25%之间，相对标准偏差（RSD）在1.23%至2.44%之间。该方法简便、快速、准确，适用于四君子丸的质量控制。
