Luk'ianchuk V V
Mikrobiol Z. 2009 Sep-Oct;71(5):48-51.
Copies of chromosome Streptomyces coelicolor A3(2) fragment (gene coding polyketide syntase of III type) were obtained to use polymerase cycle reaction. Amplified DNA was cloned in shuttle vector pWHM4 on sites for HindIII and EcoRI. The restriction analysis of plasmid DNAs of transformants has demonstrated that molecular size of cloned DNA fragment was 1134 bp.
获得了天蓝色链霉菌A3(2)染色体片段（编码III型聚酮合酶的基因）的拷贝，用于聚合酶循环反应。扩增的DNA被克隆到穿梭载体pWHM4的HindIII和EcoRI位点上。对转化体的质粒DNA进行的限制性分析表明，克隆的DNA片段的分子大小为1134 bp。
