Department of Environmental Toxicology, University of California, Davis, California 95616, USA.
J Agric Food Chem. 2010 Jun 9;58(11):6672-7. doi: 10.1021/jf1003573.
A crude methanol extract prepared from fresh broccoli sprouts was extracted with hexane, chloroform, ethyl acetate, and butanol sequentially. Residual water fraction was obtained from the residual aqueous layer. The greatest inhibition zones (>5 cm) were noted for Helicobacter pylori strain by the chloroform extract, followed by the hexane extract (5.03 cm), the ethyl acetate extract (4.90 cm), the butanol extract (3.10 cm), and the crude methanol extract (2.80 cm), whereas the residual water fraction did not show any inhibition zone. Including sulforaphane, five sulforaphane-related compounds were positively identified in the chloroform extract, of which 5-methylsulfinylpentylnitrile was found in the greatest concentration (475.7 mg/kg of fresh sprouts), followed by sulforaphane (222.6 mg/kg) and 4-methylsulfinylbutylnitrile (63.0 mg/kg). Among 18 sulforaphane and related compounds synthesized (6 amines, 6 isothiocyanates, and 6 nitriles), 2 amines, 6 isothiocyanates, and 1 nitrile exhibited >5 cm inhibitory zones for H. pylori strain. The results indicate that broccoli sprouts can be an excellent food source for medicinal substances.
从新鲜西兰花芽中制备的粗甲醇提取物依次用正己烷、氯仿、乙酸乙酯和正丁醇萃取。从残留的水层中得到残留水部分。氯仿提取物对幽门螺杆菌菌株的抑制圈最大（>5cm），其次是正己烷提取物（5.03cm）、乙酸乙酯提取物（4.90cm）、正丁醇提取物（3.10cm）和粗甲醇提取物（2.80cm），而残留水部分没有显示任何抑制圈。包括萝卜硫素在内，在氯仿提取物中鉴定出五种与萝卜硫素相关的化合物，其中 5-亚甲基亚磺酰基戊腈的浓度最高（475.7mg/kg 新鲜芽），其次是萝卜硫素（222.6mg/kg）和 4-亚甲基亚磺酰基丁腈（63.0mg/kg）。在所合成的 18 种萝卜硫素和相关化合物（6 种胺、6 种异硫氰酸酯和 6 种腈）中，有 2 种胺、6 种异硫氰酸酯和 1 种腈对幽门螺杆菌菌株的抑制圈大于 5cm。结果表明，西兰花芽可以作为药用物质的极好的食物来源。
