Catholic University of America, Department of Biomedical Engineering, 620 Michigan Avenue North East, Washington, DC 20064, USA.
J Biomed Opt. 2010 Mar-Apr;15(2):021321. doi: 10.1117/1.3333546.
We report experimentally observed ultrasound-modulated fluorescence (UMF) from a submillimeter tube filled with rhodamine B aqueous solution. The tube was submerged in water and a scattering medium. Based on the measured data, we find that the UMF signals might be generated from three mechanisms: modulation of the excitation light, modulation of the emission light, and modulation of the properties of fluorophore. In addition, a linear relationship between the UMF and the drive voltage applied to the ultrasound transducer is found.
我们实验观察到填充有罗丹明 B 水溶液的亚毫米管的超声调制荧光(UMF)。该管浸没在水中和散射介质中。根据测量数据,我们发现 UMF 信号可能源自三种机制:激发光的调制、发射光的调制和荧光团性质的调制。此外,还发现 UMF 与施加到超声换能器的驱动电压之间存在线性关系。