Institute of Molecular Biotechnology, University of Natural Resources and Life Sciences, Vienna, Vienna, Austria.
MDI Biological Laboratory, Bar Harbor, United States.
Elife. 2020 Dec 8;9:e56205. doi: 10.7554/eLife.56205.
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing mC at position C2381 on the 26S rRNA in . Here, we show that NSUN-1 is writing the second known 26S rRNA mC at position C2982. Depletion of or improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of extended lifespan. Moreover, soma-specific knockdown of reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by depletion, translation of specific mRNAs was remodeled leading to reduced production of collagens, loss of structural integrity of the cuticle, and impaired barrier function. We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal development and physiology.
我们对核糖体 RNA 修饰的文库和负责安装它们的酶的了解在不断扩展。之前，我们报道了 NSUN-5 负责在. 中在 26S rRNA 上的位置 C2381 沉积 mC。在这里，我们表明 NSUN-1 正在位置 C2982 书写第二个已知的 26S rRNA mC。 或 的耗竭提高了热耐受性并略微增加了中年时的运动能力，但只有体细胞特异性敲低 延长了寿命。此外，体细胞特异性敲低 降低了体型并损害了繁殖力，表明存在非细胞自主效应。虽然核糖体生物发生和全局蛋白质合成不受 耗竭的影响，但特定 mRNA 的翻译被重塑，导致胶原蛋白的产生减少、表皮结构完整性丧失以及屏障功能受损。我们得出结论，丧失单个 rRNA 甲基化所需的酶对生物体的发育和生理有深远而高度特异的影响。
