鸡 VIP 基因多态性与产蛋性能和就巢性状的相关性研究。

Associations between polymorphisms in the chicken VIP gene, egg production and broody traits.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangdong, China.

Br Poult Sci. 2010 Apr;51(2):195-203. doi: 10.1080/00071661003745786.

Abstract

In this study, the vasoactive intestinal peptide (VIP) gene was screened for variations by cloning and sequencing to analyse the association with chicken egg production and broodiness traits. 2. Sequencing revealed that 69 polymorphisms were found in a 9305-bp length of the chicken VIP gene, of which 39 were located in introns, 28 in 5' regulatory region, 1 in exon 6 (synonymous) and 1 in 3'-UTR. The nucleotide diversity corrected for sample size was 1.96 x 10(-3). 3. Five polymorphisms, C-3134T, "AGG" indel at -2648 to -2650, C + 338T, G + 780T and A + 4691G, were genotyped in 644 individuals of Ningdu Sanhuang chickens to evaluate their effects on egg production and broodiness traits. Marker-trait association analyses showed that the "AGG" indel was associated with total number of eggs from 90 to 300 d of age and total number of qualified eggs from 90 to 300 d of age. In contrast, C + 338T was associated with duration of broodiness.

摘要

在这项研究中，通过克隆和测序筛选血管活性肠肽 (VIP) 基因的变异，分析其与鸡产蛋和抱窝性状的关系。
测序结果显示，在鸡 VIP 基因的 9305bp 长度内发现了 69 个多态性，其中 39 个位于内含子中，28 个位于 5'调控区，1 个位于外显子 6（同义），1 个位于 3'-UTR。经样本量校正的核苷酸多样性为 1.96 x 10(-3)。
在 644 只宁都三黄鸡个体中，对 C-3134T、-2648 到-2650 处“AGG”插入缺失、C+338T、G+780T 和 A+4691G 这 5 个多态性进行了基因型分析，以评估它们对产蛋和抱窝性状的影响。标记-性状关联分析表明，“AGG”插入缺失与 90 至 300 日龄总产蛋数和 90 至 300 日龄总合格蛋数有关。相比之下，C+338T 与抱窝持续时间有关。