Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya'an, 625014, People's Republic of China.
Mol Biol Rep. 2013 Dec;40(12):7111-6. doi: 10.1007/s11033-013-2834-6. Epub 2013 Nov 6.
Luteinizing hormone/choriogonadotropin receptor (LHCGR) gene, potentially related to reproductive traits in chickens, was genotyped by using the Pooled DNA Sequencing, PCR-SSCP and Directing Sequencing techniques. 306 Erlang Mountain chickens form one line (SD03, a line that has been selected for egg quality from a local chicken breed in Sichuan province, China) were genotyped in this study. The associations between LHCGR polymorphisms and six reproductive traits [body weight at first egg (BWAFE), weight of first egg, age at first egg (AFE), number of eggs at 300 days of age (EN), body weight at 300 days of age and egg weight at 300 days of age (EWTA)] were estimated using the one-way analysis of variance method. Results showed that SNP +G4058A and SNP +T4099G of the LHCGR gene were significantly associated with BWFE and AFE. Birds with the AG genotype for the +G4058A SNP exhibited shorter AFE (P < 0.05) and greater EN than those of the GG and AA genotypes, suggesting a balancing selection (overdominance); the effect of allele C in SNP +C3021T and allele C in SNP +T4490C on EN and AFE is additive and may reflect the influence of positive selection. These alleles have promise as genetic markers for future marker-assisted selection.
黄体生成素/绒毛膜促性腺激素受体 (LHCGR) 基因可能与鸡的繁殖性状有关，本研究采用 Pooled DNA 测序、PCR-SSCP 和直接测序技术对其进行了基因分型。对 306 只二郎山山地鸡（SD03 是从中国四川省的一个本地鸡种中选育出来的蛋品质鸡系）进行了基因分型。采用单因素方差分析方法，对 LHCGR 多态性与 6 个繁殖性状（首次产蛋体重（BWAFE）、首次蛋重、首次产蛋日龄（AFE）、300 日龄产蛋数（EN）、300 日龄体重和 300 日龄蛋重（EWTA））之间的相关性进行了估计。结果表明，LHCGR 基因的 SNP +G4058A 和 SNP +T4099G 与 BWFE 和 AFE 显著相关。+G4058A SNP 的 AG 基因型的鸡具有较短的 AFE（P < 0.05）和较高的 EN，表明存在平衡选择（超显性）；SNP +C3021T 中的等位基因 C 和 SNP +T4490C 中的等位基因 C 对 EN 和 AFE 的影响是累加的，可能反映了正选择的影响。这些等位基因有望成为未来标记辅助选择的遗传标记。
