血府逐瘀汤对内皮细胞系ECV304血管生成基因调控的作用
[Effect of xuefu zhuyu decoction in inducing angiogenesis gene regulation of endothelial cell line ECV304].
作者信息
Gao Dong, Chen Wen-yuan, Wu Li-ya
机构信息
Fujian College of Traditional Chinese Medicine, Fuzhou.
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Feb;30(2):153-6.
OBJECTIVE
To study the acting mechanism of endothelial cell line ECV304 in regulating angiogenesis induced by Xuefu Zhuyu Decoction (XFZY).
METHODS
The angiogenesis effect of XFZY-contained serum (XFZY-CS) was confirmed by observing its impact on proliferation, cell cycle, migration of ECV304 and on vascular neogenesis in vitro. Then the effect of XFZY on various angiogenesis controlling factors was analyzed with gene chip microarray technique.
RESULTS
Treatment of XFZY-CS in 2.5% concentration for 48 h showed evident actions of enhancing ECV304 activity, increasing cell numbers of S phase, inducing cell migration and promoting the in vitro angiogenesis. Meanwhile, expressions of four angiogenesis controlling genes were up-regulated and 10 were down-regulated.
CONCLUSION
The angiogenesis mechanism of ECV304 induced by XFZY is complex, it shows a multi-pathway and multi-target feature.
目的
研究血管内皮细胞系ECV304在调控血府逐瘀汤（XFZY）诱导血管生成中的作用机制。
方法
通过观察含XFZY血清（XFZY-CS）对ECV304增殖、细胞周期、迁移及体外血管新生的影响，证实其血管生成作用。然后采用基因芯片微阵列技术分析XFZY对各种血管生成调控因子的影响。
结果
2.5%浓度的XFZY-CS处理48 h显示出明显增强ECV304活性、增加S期细胞数量、诱导细胞迁移及促进体外血管生成的作用。同时，4个血管生成调控基因表达上调，10个下调。
结论
XFZY诱导ECV304血管生成的机制复杂，呈现多途径、多靶点特征。
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