雪府逐瘀汤对血管生成调控作用的基因芯片分析。
A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction on endothelial cells.
机构信息
Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Chin J Integr Med. 2012 Jul;18(7):502-6. doi: 10.1007/s11655-012-1143-6. Epub 2012 Jul 7.
Abstract
OBJECTIVE
To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction () on the endothelial cell line ECV304.
METHODS
ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction-containing serum (XFZYD-CS) for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT), fluorescence activating cell sorter (FACS), migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray.
RESULTS
At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed.
CONCLUSION
XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.
摘要
目的
研究血府逐瘀汤（）对血管内皮细胞系 ECV304 的血管生成调节机制。
方法
用含 2.5%血府逐瘀汤的血清（XFZYD-CS）处理 ECV304 细胞 24、48 或 72 小时。噻唑蓝溴化四唑（MTT）、荧光激活细胞分选（FACS）、迁移、黏附和体外管形成试验用于在 3 个时间点确认 XFZYD 的血管生成作用。实时聚合酶链反应（RT-PCR）超微阵列用于在 3 个时间点分析血管生成调节剂谱。
结果
48 小时时，XFZYD-CS 诱导 ECV304 细胞活力、S 期细胞数量、迁移、黏附和管形成显著提高。在 24 小时和 72 小时，仅细胞迁移增加。微阵列结果显示 27 个血管生成相关基因的表达发生改变。
结论
XFZYD-CS 处理诱导 ECV304 细胞血管生成，48 小时时出现显著的细胞变化，24 小时时出现早期基因变化。
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