[血府逐瘀汤对内皮祖细胞功能的影响]

[Effects of Xuefu Zhuyu decoction on EPCs function].

作者信息

Gao Dong, Wu Li-Ya, Jiao Yu-Huan, Chen Wen-Yuan, Chen Yan, Lu Bo, Song Jun, Chen Ke-Ji

机构信息

College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China.

出版信息

Zhong Yao Cai. 2010 Jul;33(7):1129-32.

PMID:21137372

Abstract

OBJECTIVE

To observe the effect on EPCs function by Xuefu Zhuyu Decoction.

METHODS

After induced by serial concentrations of Xuefu Zhuyu Decoction-contained serum (XZDCS) and blank serum, EPCs proliferation, migration, adhesion and uptake function were detected by MTT, Boyden chamber, adhesion and uptake of ac-LDL respectively.

RESULTS

Compared with the control group, 15% and 10% XZDCS could elevate the cell regeneration for 24 h and 48 h respectively. Both concentrations could improve the EPCs migration and adhesion for all 24, 48, 72 h and uptake of ac-LDL only 48 h. But 10% XZDCS could last effect on uptake of ac-LDL to 72 h and 5% XZDCS converted the inhibition of cell adhesion in the first 24 h to promotion in the next 48 - 72 h.

CONCLUSION

Xuefu Zhuyu Decoction could induce EPCs differentiation into EC to angiogenesis by promoting its proliferation, migration, adhesion and uptake function.

摘要

目的

观察血府逐瘀汤对内皮祖细胞（EPCs）功能的影响。

方法

用含不同浓度血府逐瘀汤血清（XZDCS）及空白血清诱导EPCs后，分别采用MTT法、Boyden小室法、黏附实验及乙酰化低密度脂蛋白（ac-LDL）摄取实验检测EPCs的增殖、迁移、黏附及摄取功能。

结果

与对照组相比，15%和10%的XZDCS分别在24 h和48 h时可提高细胞再生能力。两种浓度均能在24、48、72 h时改善EPCs的迁移和黏附能力，且仅在48 h时能促进ac-LDL摄取。但10%的XZDCS对ac-LDL摄取的作用可持续至72 h，5%的XZDCS可使细胞黏附在最初24 h的抑制作用转变为随后48 - 72 h的促进作用。