Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108, China.
Chin J Integr Med. 2010 Feb;16(1):50-3. doi: 10.1007/s11655-010-0050-y. Epub 2010 Feb 4.
To observe the effect of Xuefu Zhuyu Decoction ()-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.
Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.
After one day, EPCs exposed to the serum containing 5%, 10% and 15% XFZYD-CS formed typical tubes or vessel networks. The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.
XFZYD-CS could induce EPC angiogenesis and hasten tube formation, especially in capillary vessels. The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.
观察血府逐瘀汤含药血清（XFZYD-CS）对体外血管内皮祖细胞（EPC）管腔形成的影响。
采用密度梯度离心法分离大鼠骨髓单个核细胞，采用差速贴壁法分离 EPC，并倒置显微镜观察 XFZYD-CS 对 EPC 管腔形成的影响。
XFZYD-CS 含药血清浓度为 5%、10%、15%时，EPC 培养 1 天后即可形成典型的管腔或血管网络，管腔形成时间比对照组提前 2 天，且血清组中大多数管腔的大小均小于对照组。
XFZYD-CS 能诱导 EPC 血管生成并促进管腔形成，尤其促进毛细血管的生成。本研究为血府逐瘀汤治疗缺血性疾病的合理性提供了实验依据。
