Jongen M J, Engel R, Leenheers L H
Department of Occupational Toxicology, Medical Biological Laboratory, Rijswijk, The Netherlands.
J Anal Toxicol. 1991 Jan-Feb;15(1):30-4. doi: 10.1093/jat/15.1.30.
An HPLC method was developed for application in the measurement of occupational exposure to the pesticide chlorothalonil. In addition, sampling methods were validated for the determination of exposure to chlorothalonil in the greenhouse culturing of carnations. Procedures for sampling of the inspirable fraction of aerosols, for the determination of hand contamination by hand rinse and the use of cotton gloves, and for the determination of dislodgable chlorothalonil residues on carnation leaves were validated. Normal phase HPLC with hexane-dioxane as the eluent and UV detection at either 254 or 325 nm appeared suitable for the determination of chlorothalonil in the described matrices. The limit of detection, after concentration on SepPak C18 cartridges was approximately 0.5 micrograms/L. Linear calibration curves were obtained in concentration ranges from 0.5 microgram/L to 100 mg/L. In general, no interferences were noticed in the analysis of the matrices. However, cotton gloves for determination of hand contamination had to be washed before use because they contained interfering material, and in the case of air sampling, glass fiber filters for air sampling appeared to degrade chlorothalonil very rapidly. Therefore, cellulose filters were used for collection of the inspirable fraction of aerosols containing chlorothalonil.
开发了一种高效液相色谱（HPLC）方法，用于测定职业接触农药百菌清的情况。此外，还对康乃馨温室栽培中百菌清接触量的测定采样方法进行了验证。对可吸入气溶胶部分的采样程序、通过手洗和使用棉手套测定手部污染的程序以及测定康乃馨叶片上可去除百菌清残留的程序进行了验证。以正己烷 - 二氧六环为洗脱剂、在254或325 nm处进行紫外检测的正相HPLC法似乎适用于测定上述基质中的百菌清。在SepPak C18柱上浓缩后，检测限约为0.5微克/升。在0.5微克/升至100毫克/升的浓度范围内获得了线性校准曲线。一般来说，在基质分析中未发现干扰。然而，用于测定手部污染的棉手套在使用前必须清洗，因为它们含有干扰物质，并且在空气采样的情况下，用于空气采样的玻璃纤维滤膜似乎会非常迅速地降解百菌清。因此，使用纤维素滤膜收集含有百菌清的可吸入气溶胶部分。
