Al-Harithy Rowyda N, Al-Ghafari Ayat B
Biochemistry Department, King Abdul- Aziz University, PO Box 40288, Jeddah 21499, Kingdom of Saudi Arabia.
Saudi Med J. 2010 May;31(5):495-500.
To determine the relationship between resistin gene RETN C-180G variant and circulating resistin concentration in Saudi colon cancer patients.
This case-control study was conducted in the Biochemistry Department, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia from April 2009 to December 2009. The serum concentration had been measured with enzyme-linked immunosorbent assay in 60 colon cancer patients and in 60 controls matched in gender and age. The single nucleotide polymorphism SNP C-180G was genotyped using polymerase chain reaction PCR and restriction fragment length polymorphism RFLP techniques.
We observed a significantly higher serum resistin level in colon cancer group compared with control group 19.44 +/- 8.46 versus 5.45 +/- 2.73 ng/ml; p=0.0001, with significant p=0.03 higher levels showed in women than in men in patients and controls. In patients, the heterozygous CG and homozygous GG genotype carriers showed higher p=0.08 levels of serum resistin compared to CC homozygous. This difference was not observed p=0.78 among SNP C-180G genotypes in control group.
Our result showed no association between the C-180G SNP and the serum resistin concentrations and suggests that the high resistin level in colon cancer patients may play an important role in colon cancer development.
确定沙特结肠癌患者中抵抗素基因RETN C-180G变异与循环抵抗素浓度之间的关系。
本病例对照研究于2009年4月至2009年12月在沙特阿拉伯王国吉达阿卜杜勒阿齐兹国王大学的生物化学系进行。采用酶联免疫吸附测定法测量了60例结肠癌患者和60例年龄及性别匹配的对照者的血清浓度。使用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）技术对单核苷酸多态性SNP C-180G进行基因分型。
我们观察到结肠癌组的血清抵抗素水平显著高于对照组（19.44±8.46对5.45±2.73 ng/ml；p=0.0001），患者和对照组中女性的水平均显著高于男性（p=0.03）。在患者中，杂合子CG和纯合子GG基因型携带者的血清抵抗素水平高于CC纯合子（p=0.08）。在对照组的SNP C-180G基因型之间未观察到这种差异（p=0.78）。
我们的结果显示C-180G SNP与血清抵抗素浓度之间无关联，并表明结肠癌患者中高抵抗素水平可能在结肠癌发展中起重要作用。
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