Gorgian Mohammadi Masumeh, Hedayati Mehdi, Zarghami Nosratollah, Ghaemmaghami Sara, Mohaddes Mojtaba
Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran ; Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Iran J Cancer Prev. 2013 Summer;6(3):165-9.
Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line.
Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion.
Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed.
Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer.
脂肪组织具有内分泌器官的特征，可释放多种脂肪细胞特异性因子，即脂肪细胞因子。最近有研究表明，脂肪细胞因子可能在某些癌症的发病机制和进展中发挥作用，尤其是在胃癌中。本研究旨在调查胃癌细胞系中内脂素和抵抗素的内源性和/或外源性表达。
通过细胞培养和半定量逆转录聚合酶链反应来检测胃癌细胞系中抵抗素和内脂素的mRNA和蛋白质表达。采用酶联免疫吸附测定法检测细胞裂解液和细胞培养上清液中抵抗素和内脂素的蛋白质表达及分泌情况。
发现人胃癌细胞系（AGS细胞系）表达内脂素mRNA和蛋白质，但不表达抵抗素mRNA和蛋白质。
内脂素在AGS人胃癌细胞中内源性表达。相反，抵抗素不表达。本研究结果表明，实际样本中脂肪细胞因子蛋白的表达可能是胃癌的一个生物标志物。
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