[从医疗机构环境及患者中分离出的铜绿假单胞菌菌株的分子遗传学分析]
[Molecular genetic analysis of Pseudomonas aeruginosa strains isolated from environment and patients in health care facilities].
作者信息
Antonov V A, Altukhova V V, Savchenko S S, Tkachenko G A, Zamaraev V S, Zhukova S I, Kramar' O G, Matveeva N L, Ostrovskiĭ O V, Dudchenko G P
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2010 Mar-Apr(2):8-13.
AIM
Analysis of genetic heterogeneity of Pseudomonas aeruginosa strains isolated in and out of hospitals.
MATERIALS AND METHODS
To study the genetic diversity of 36 strains of P. aeruginosa plasmid analysis, random amplified polymorphic DNA (RAPD) technique as well as polymerase chain reaction for detection of virulence genes algD, lasB, toxA, plcH, plcN, exoS, nan1, and nan2.
RESULTS
Epidemically important strains were found in different ecological niches. It was shown that these virulence factors could play important roles in pathogenesis of infection.
CONCLUSION
RAPD technique was effective for analysis of P. aeruginosa isolates. Number of studied typing bands differed between related isolates for each random primer.
目的
分析医院内外分离的铜绿假单胞菌菌株的遗传异质性。
材料与方法
为研究36株铜绿假单胞菌的遗传多样性，采用质粒分析、随机扩增多态性DNA（RAPD）技术以及聚合酶链反应检测毒力基因algD、lasB、toxA、plcH、plcN、exoS、nan1和nan2。
结果
在不同生态位中发现了具有流行病学重要性的菌株。结果表明，这些毒力因子在感染发病机制中可能起重要作用。
结论
RAPD技术对分析铜绿假单胞菌分离株有效。每个随机引物的相关分离株之间研究的分型条带数量不同。
Zotero 插件