Faraji Fatemeh, Mahzounieh Mohammadreza, Ebrahimi Azizollah, Fallah Fatemeh, Teymournejad Omid, Lajevardi Behnaz
Dept. of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Dept. of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Microb Pathog. 2016 Oct;99:1-4. doi: 10.1016/j.micpath.2016.07.013. Epub 2016 Jul 25.
Pseudomonas aeruginosa possesses various virulence factors which contribute to the bacterial invasion and toxicity. Moreover, children suffered from Cystic Fibrosis (CF) and burn wounds are at a high risk of various bacterial infections. The aim of this study was to determine the prevalence of virulent genes in P. aeruginosa isolated from children with CF and burn wounds and comparing their virulence genes to figure out the role of every virulent factor in the infections. P. aeruginosa were isolated from sputum, oropharyngeal swabs, and broncho-alveolar lavage (BAL) specimens from CF and burn wounds between June 2013 and June 2014 in Tehran's hospitals. Bacterial genomic DNAs were extracted and uniplex, duplex and multiplex PCR were performed for detection of toxA, algD and plcN, exoS, lasB, plcH genes, respectively. The prevalence rate of virulence genes in P. aeruginosa isolated from CF was; toxA (63.1%), algD (64.6%), plcH (87.7%), plcN (60%), lasB (95.4%) and exoS (70.8%) and virulence genes in P. aeruginosa from burn patients were: toxA (36.9%), algD (70.1%), plcH (79%), plcN (63.1%), lasB (82%) and exoS (21.1%). The prevalence of three virulent genes in P. aeruginosa was higher in CF comparing to burn wound infections. We found that the number of toxA, lasB and exoS were significantly higher in the bacteria which were isolated from children with CF. This finding shows that these virulence factors play an important role in CF infections by P. aeroginosa.
铜绿假单胞菌拥有多种毒力因子，这些因子有助于细菌的侵袭和毒性。此外，患有囊性纤维化（CF）和烧伤创面的儿童极易发生各种细菌感染。本研究的目的是确定从患有CF和烧伤创面的儿童中分离出的铜绿假单胞菌中毒力基因的流行情况，并比较它们的毒力基因，以弄清楚每种毒力因子在感染中的作用。2013年6月至2014年6月期间，从德黑兰医院的CF和烧伤创面患者的痰液、口咽拭子和支气管肺泡灌洗（BAL）标本中分离出铜绿假单胞菌。提取细菌基因组DNA，分别进行单重、双重和多重PCR检测toxA、algD和plcN、exoS、lasB、plcH基因。从CF患者中分离出的铜绿假单胞菌中毒力基因的流行率为：toxA（63.1%）、algD（64.6%）、plcH（87.7%）、plcN（60%）、lasB（95.4%）和exoS（70.8%）；从烧伤患者中分离出的铜绿假单胞菌中毒力基因的流行率为：toxA（36.9%）、algD（70.1%）、plcH（79%）、plcN（63.1%）、lasB（82%）和exoS（21.1%）。与烧伤创面感染相比，CF患者中铜绿假单胞菌的三种毒力基因流行率更高。我们发现，从CF患儿中分离出的细菌中，toxA、lasB和exoS的数量明显更高。这一发现表明，这些毒力因子在铜绿假单胞菌引起的CF感染中起重要作用。
