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编码细胞色素b2的酿酒酵母CYB2基因的复杂转录调控：CYP1（HAP1）激活剂与CYB2上游激活位点UAS1-B2结合。

Complex transcriptional regulation of the Saccharomyces cerevisiae CYB2 gene encoding cytochrome b2: CYP1(HAP1) activator binds to the CYB2 upstream activation site UAS1-B2.

作者信息

Lodi T, Guiard B

机构信息

Institute of Genetics, University of Parma, Italy.

出版信息

Mol Cell Biol. 1991 Jul;11(7):3762-72. doi: 10.1128/mcb.11.7.3762-3772.1991.

DOI:10.1128/mcb.11.7.3762-3772.1991
PMID:2046677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361146/
Abstract

Expression of the Saccharomyces cerevisiae gene encoding cytochrome b2 (EC 1.2.2.3), CYB2, was investigated by direct analysis of mRNA transcripts and by measurement of the expression of lacZ fused to the CYB2 control regions. These studies indicated that regulation of the CYB2 gene is subject to several metabolic controls at the transcriptional level: inhibition due to glucose fermentation, induction by lactate, and inhibition in anaerobiosis or in absence of heme biosynthesis. Furthermore, we have shown that the CYB2 promoter contains one cis negative regulatory region and two heme-dependent positive regions, one of which is controlled by the transcriptional regulator CYP1 (HAP1) which is involved in the modulation of the expression of several oxygen-regulated genes. The CYP1 (HAP1)-binding sequence was located by gel retardation and DNase I footprinting experiments and compared with the binding sequences previously characterized in detail (UAS1CYC1, UAS'CYP3 (CYC7), and UASCTT1).

摘要

通过对信使核糖核酸转录本的直接分析以及对与CYB2调控区融合的lacZ表达的测量，研究了酿酒酵母中编码细胞色素b2（EC 1.2.2.3）的基因CYB2的表达。这些研究表明，CYB2基因的调控在转录水平上受到多种代谢控制：因葡萄糖发酵而受到抑制，因乳酸而被诱导，以及在无氧条件下或血红素生物合成缺失时受到抑制。此外，我们已经表明CYB2启动子包含一个顺式负调控区和两个血红素依赖性正调控区，其中一个由转录调节因子CYP1（HAP1）控制，该因子参与调控多个氧调节基因的表达。通过凝胶阻滞和DNase I足迹实验确定了CYP1（HAP1）结合序列，并与先前详细表征的结合序列（UAS1CYC1、UAS'CYP3（CYC7）和UASCTT1）进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/90e664e0459c/molcellb00031-0380-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/0e005fefe3eb/molcellb00031-0376-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/3988bdbd1b71/molcellb00031-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/ed5632ad2e30/molcellb00031-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/1a84a5cbbfa4/molcellb00031-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/498cd1451688/molcellb00031-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/90e664e0459c/molcellb00031-0380-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/0e005fefe3eb/molcellb00031-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/05f73ed97fa9/molcellb00031-0376-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/3988bdbd1b71/molcellb00031-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/ed5632ad2e30/molcellb00031-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/1a84a5cbbfa4/molcellb00031-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/498cd1451688/molcellb00031-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e62/361146/90e664e0459c/molcellb00031-0380-b.jpg

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