利用富含精油的植物材料固定化罗奇氏菌 L4 对偶氮氯苯的共代谢降解。
Cometabolic degradation of trichloroethene by Rhodococcus sp. strain L4 immobilized on plant materials rich in essential oils.
机构信息
Department of Microbiology, Chulalongkorn University, Patumwan, Bangkok, Thailand.
出版信息
Appl Environ Microbiol. 2010 Jul;76(14):4684-90. doi: 10.1128/AEM.03036-09. Epub 2010 May 14.
Abstract
The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 muM TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.
摘要
通过添加诱导物（如枯茗醇、柠檬烯或枯茗醛）可以克服 Rhodococcus sp. L4 共代谢降解三氯乙烯（TCE）时酶活性丧失的限制。或者，可以将 Rhodococcus sp. L4 固定在含有这些诱导物的植物材料上。枯茗籽是最适合的固定化材料，固定化细胞可以耐受高达 68 μM 的 TCE 并持续降解 TCE。在没有 TCE 的情况下，在无机盐培养基中孵育可以使部分失活的固定化细胞重新激活。这些发现表明，将 Rhodococcus sp. L4 固定在富含精油的植物材料上是一种很有前途的方法，可用于有效共代谢降解 TCE。
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Biodegradation. 2009 Apr;20(2):281-91. doi: 10.1007/s10532-008-9220-4. Epub 2008 Sep 23.
2
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J Hazard Mater. 2007 Sep 30;148(3):660-70. doi: 10.1016/j.jhazmat.2007.03.030. Epub 2007 Mar 14.
3
Inhibition, Inactivation, and Recovery of Ammonia-Oxidizing Activity in Cometabolism of Trichloroethylene by Nitrosomonas europaea.
Appl Environ Microbiol. 1995 Apr;61(4):1480-7. doi: 10.1128/aem.61.4.1480-1487.1995.
4
Aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by rhodococcus species.
Appl Environ Microbiol. 1994 Feb;60(2):542-8. doi: 10.1128/aem.60.2.542-548.1994.
5
Attachment stimulates exopolysaccharide synthesis by a bacterium.
Appl Environ Microbiol. 1993 Oct;59(10):3280-6. doi: 10.1128/aem.59.10.3280-3286.1993.
6
Degradation of carbendazim and 2,4-dichlorophenoxyacetic acid by immobilized consortium on loofa sponge.
J Biosci Bioeng. 2004;98(1):28-33. doi: 10.1016/S1389-1723(04)70238-8.
7
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Bioresour Technol. 2006 Jan;97(1):32-8. doi: 10.1016/j.biortech.2005.02.031. Epub 2005 Apr 12.
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