Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, 6431 Fannin, Houston, TX 77030, USA.
Am J Clin Pathol. 2010 Jun;133(6):878-83. doi: 10.1309/AJCPN6MWATQ3SZTC.
A recent report indicated significant interference of naproxen with total bilirubin measurement when using the Jendrassik and Grof method. We explored the possibility of avoiding this interference by using a different method. We observed that naproxen has no effect on total bilirubin measurement by any method, but O-desmethylnaproxen (the naproxen metabolite) interfered significantly with total bilirubin assays using the Jendrassik and Grof method (Beckman Coulter [Brea, CA] and Siemens [Tarrytown, NY] bilirubin method). The Roche (Indianapolis, IN) bilirubin assay based on a different method was not affected. When serum samples of 9 patients receiving naproxen were analyzed for total bilirubin level, we observed good correlation in total bilirubin values measured by all 3 methods in 7 of 9 patients with therapeutic naproxen concentrations. In contrast, in 2 patients with elevated naproxen levels, the total bilirubin values observed by using the Beckman and Siemens methods were significantly higher than the value obtained by the Roche assay. The naproxen metabolite interferes with assays based on the Jendrassik and Grof method but not with the Roche bilirubin assay.
最近的一份报告表明，使用 Jendrassik 和 Grof 法时，萘普生会对总胆红素的测量产生显著干扰。我们探讨了使用不同方法避免这种干扰的可能性。我们观察到，萘普生不会影响任何方法测量的总胆红素，但 O-去甲基萘普生（萘普生的代谢物）会显著干扰 Jendrassik 和 Grof 法（贝克曼库尔特[贝克斯菲尔德，加利福尼亚州]和西门子[塔里敦，纽约]胆红素法）测定总胆红素。罗氏（印第安纳波利斯，印第安纳州）基于不同方法的胆红素测定不受影响。当分析 9 名接受萘普生治疗的患者的血清样本的总胆红素水平时，我们观察到在 7 名患者中，所有 3 种方法测量的总胆红素值与治疗性萘普生浓度有很好的相关性。相比之下，在 2 名萘普生水平升高的患者中，使用贝克曼和西门子方法观察到的总胆红素值明显高于罗氏检测法获得的值。萘普生代谢物会干扰基于 Jendrassik 和 Grof 法的测定，但不会干扰罗氏胆红素测定。
