Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel.
Hum Reprod. 2010 Jul;25(7):1708-12. doi: 10.1093/humrep/deq121. Epub 2010 May 15.
Ewing sarcoma (EWS) is a highly metastatic malignancy in young patients. Ovarian cryopreservation is often an option for fertility preservation in cancer patients of reproductive age, specifically in minors. Thus, the possibility of ovarian involvement in EWS needs to be elucidated.
Eight patients aged 13-20 years with EWS participated in the study. Ovarian samples were fixed and prepared for light microscopy, and frozen in liquid nitrogen for RNA extraction followed by RT-PCR. Histological studies, including immunostaining for the adhesion receptor CD99, were used to detect histopathological features. Sensitive molecular methods were used to detect translocations causing the formation of tumor-specific EWS-Friend leukemia virus integration site 1 fusion gene (EWS-FLI1).
In seven patients, there was no evidence of EWS in the ovaries from pathological/molecular studies. However, in one patient, the RT-PCR showed the EWS translocation, although there was no pathological evidence.
Ovarian involvement is possible in EWS. Therefore, in patients with EWS ovarian tissue should be examined for traces of malignancy at both the pathological and molecular levels prior to the grafting of cryopreserved tissue in order to minimize the risk of reseeding the cancer.
尤因肉瘤（EWS）是一种高度转移性的恶性肿瘤，常见于年轻患者。对于有生育需求的生殖年龄期癌症患者，尤其是未成年患者，常常选择进行卵巢组织冷冻保存。因此，有必要明确 EWS 是否会累及卵巢。
本研究纳入了 8 名 13-20 岁的 EWS 患者。对患者的卵巢样本进行固定和光镜检查，液氮冻存用于提取 RNA 并进行 RT-PCR。通过组织学研究，包括黏附受体 CD99 的免疫染色，来检测组织病理学特征。采用敏感的分子方法检测导致形成肿瘤特异性 EWS-Friend 白血病病毒整合位点 1 融合基因（EWS-FLI1）的易位。
在 7 名患者中，病理/分子研究均未发现卵巢中存在 EWS。然而，在 1 名患者中，尽管没有病理学证据，但 RT-PCR 显示存在 EWS 易位。
EWS 可能累及卵巢。因此，在对冷冻保存的组织进行移植之前，应对 EWS 患者的卵巢组织进行病理和分子水平的恶性肿瘤检测，以最大程度降低癌症种植的风险。
