Tateishi K, Funakoshi A, Jimi A, Funakoshi S, Tamamura H, Yajima H, Matsuoka Y
First Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Gastroenterology. 1989 Nov;97(5):1313-8. doi: 10.1016/0016-5085(89)91705-8.
High levels of pancreastatinlike immunoreactivity were detected in the plasma (2.9 pmol/ml, greater than 200-fold the normal level), pancreas (2.9 nmol/g wet wt, greater than 450-fold the normal level), and liver (1.6 nmol/g wet wt) of a patient with pancreatic insulinoma with metastasis to the liver by a sensitive and specific radioimmunoassay for human pancreastatin. Antiserum was produced against the C-terminal fragment of human pancreastatin-(24-52), which was synthesized according to the sequence of human chromogranin A corresponding to that of pancreastatin. With the antiserum, intense immunocytochemical staining was detected in the tumors. Sephadex G-50 gel filtration showed that the tumors and plasma contained two molecular forms of pancreastatinlike immunoreactivity--a molecular form coeluted with synthetic human pancreastatin-52 and a larger molecular form (Mr approximately 12,000-15,000). The smaller form eluted in the same position as synthetic human pancreastatin-52 on reverse-phase high-performance liquid chromatography.
通过一种针对人胰抑素的灵敏且特异的放射免疫测定法，在一名发生肝转移的胰腺胰岛素瘤患者的血浆（2.9皮摩尔/毫升，高于正常水平200多倍）、胰腺（2.9纳摩尔/克湿重，高于正常水平450多倍）和肝脏（1.6纳摩尔/克湿重）中检测到高水平的胰抑素样免疫反应性。针对人胰抑素-(24 - 52)的C末端片段制备了抗血清，该片段是根据与人嗜铬粒蛋白A中对应于胰抑素的序列合成的。使用该抗血清，在肿瘤中检测到强烈的免疫细胞化学染色。葡聚糖凝胶G - 50凝胶过滤显示，肿瘤和血浆中含有两种胰抑素样免疫反应性分子形式——一种与合成的人胰抑素-52共洗脱的分子形式和一种较大的分子形式（分子量约为12,000 - 15,000）。较小的形式在反相高效液相色谱上与合成的人胰抑素-52在相同位置洗脱。
