[慢性快速眼动睡眠剥夺对大鼠能量代谢及血清FT3、FT4水平的影响]

[The effects of chronic rapid eye movement sleep deprivation on energy metabolism and FT3, FT4 level in serum of rats].

作者信息

Zeng Rong, Shao Shu-li, Xu Xin-jun, Zhang Wei-wei, Si Da-lei

机构信息

College of Life Seience and Engineering, Qiqihaer University, Qiqihaer 161006, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Feb;26(1):105-8.

PMID:20476580

Abstract

OBJECTIVE

To study the effect of chronic rapid eye movement sleep deprivation on energy metabolism, FT3, FT4 in serum.

METHODS

Rapid eye movement sleep deprivation of rats were deprived by flower pot, and then the energy metabolism were detected. The FT3, FT4 level in serum was determined by radioimmunoassay kit.

RESULTS

Rats after sleep deprivation displayed food intake increased from (75.06 +/- 25.37)g/(d x kg) to (122.30 +/- 20.43)g/(d x kg), body weight substantially decreased from (360.89 +/- 43.01) g to (295.97 +/- 37.95) g, body temperature from (37.62 +/- 1.12) degrees C up to the first (39.00 +/- 0.87) degrees C and then reduced to (37.72 +/- 0.84) degrees C, the basal metabolism rate increased significantly from (1.69 +/- 0.36) mlO2/(g x h) to (2.40 +/- 0.09) mlO2/(g x h), compared with the control group( P < 0.05). Sleep deprivation also resulted significantly lower serum thyroxine levels in comparison with the control, serum free triiodothyronine (FT3) level reduced from (3.38 +/- 0.88) pmol/L to (2.38 +/- 0.83) pmol/L, then free thyroxine(FT4) decreased from (14.62 +/- 3.62) pmol/L to (8.26 +/- 2.80) pmol/L (P < 0.05).

CONCLUSION

Rapid eye movement sleep deprivation can change energy metabolism remarkable, as well as the alteration of FT3, FT4 levels in serum.

摘要

目的

研究慢性快速眼动睡眠剥夺对能量代谢及血清中FT3、FT4的影响。

方法

采用花盆法剥夺大鼠快速眼动睡眠，然后检测能量代谢情况。采用放射免疫分析试剂盒测定血清中FT3、FT4水平。

结果

睡眠剥夺后的大鼠进食量从（75.06±25.37）g/（d×kg）增加至（122.30±20.43）g/（d×kg），体重从（360.89±43.01）g大幅降至（295.97±37.95）g，体温先从（37.62±1.12）℃升至（39.00±0.87）℃，然后降至（37.72±0.84）℃，基础代谢率从（1.69±0.36）mlO2/（g×h）显著升至（2.40±0.09）mlO2/（g×h），与对照组相比（P<0.05）。睡眠剥夺还导致血清甲状腺素水平显著低于对照组，血清游离三碘甲状腺原氨酸（FT3）水平从（3.38±0.88）pmol/L降至（2.38±0.83）pmol/L，游离甲状腺素（FT4）从（14.62±3.62）pmol/L降至（8.26±2.80）pmol/L（P<0.05）。