Kanca H, Walter I, Miller I, Schäfer-Somi S, Izgur H, Aslan S
Faculty of Veterinary Medicine, Department of Obstetrics and Gynaecology, University of Ankara, Ankara, Turkey.
Reprod Domest Anim. 2011 Apr;46(2):197-204. doi: 10.1111/j.1439-0531.2010.01619.x.
Aim of this study was to determine the intrauterine activity of matrix metalloproteinases (MMP)-2 and -9 after cessation of the local effect of progesterone. For this purpose, pregnancy was terminated in 10 bitches at mid-gestation with the progesterone receptor antagonist aglepristone (10 mg/kg body weight, sc, Alizine®; Virbac, France) at two subsequent days (group IRA = induced resorption/abortion). The IRA group was divided into two subgroups (Group I, n = 5, days 25-35 of pregnancy; group II, n = 5, days 36-45). Five further bitches were introduced with beginning abortion (group SRA = spontaneous resorption/abortion). Seven healthy bitches between day 25 and 45 of gestation served as controls. After ovariohysterectomy at the end of abortion and between days 25 and 45 of gestation, respectively, the distribution and activity of collagenases were investigated by immunohistochemistry and gelatin zymography. At placental sites, MMP-2 activity in the endometrium was significantly lower in IRA groups than in the SRA group (33.7 ± 11.8% and 39.3 ± 5.4% vs 52.2 ± 10.2%, p < 0.05); however, MMP-2 expression was lowest in the control group (control: 21.4 ± 6.3%; p < 0.01) and similarly in the myometrium (controls: 13.1 ± 2.5%; p < 0.05). MMP-9 activity was also lower in the endometrium and myometrium of the control group in comparison to SRA and IRA groups (11.8 ± 3.2%; p < 0.01 and 28.4 ± 32.8%; p < 0.05). At interplacental sites, the amount of active collagenases in the myometrium was significantly lower in the control group. It is concluded that the blockade of the biological progesterone effect was associated with an increase in activity of both collagenases.
本研究的目的是确定孕酮局部作用停止后基质金属蛋白酶（MMP）-2和-9的子宫内活性。为此，在妊娠中期，于随后两天给10只母犬注射孕酮受体拮抗剂阿利司酮（10mg/kg体重，皮下注射，Alizine®；法国维克公司）终止妊娠（IRA组=诱导吸收/流产）。IRA组分为两个亚组（I组，n=5，妊娠第25 - 35天；II组，n=5，妊娠第36 - 45天）。另外5只母犬自然流产（SRA组=自然吸收/流产）。7只妊娠第25至45天的健康母犬作为对照。分别在流产结束时以及妊娠第25至45天进行卵巢子宫切除术后，通过免疫组织化学和明胶酶谱法研究胶原酶的分布和活性。在胎盘部位，IRA组子宫内膜中MMP-2活性显著低于SRA组（分别为33.7±11.8%和39.3±5.4%，而SRA组为52.2±10.2%，p<0.05）；然而，MMP-2表达在对照组中最低（对照组：21.4±6.3%；p<0.01），在子宫肌层中情况类似（对照组：13.1±2.5%；p<0.05）。与SRA组和IRA组相比，对照组子宫内膜和子宫肌层中MMP-9活性也较低（分别为11.8±3.2%；p<0.01和28.4±32.8%；p<0.05）。在胎盘间部位，对照组子宫肌层中活性胶原酶的量显著较低。结论是，孕酮生物学效应的阻断与两种胶原酶活性增加有关。
