Institute of Environmental Science and Research Ltd, Kenepuru Science Centre, Porirua, New Zealand.
Epidemiol Infect. 2011 Mar;139(3):464-71. doi: 10.1017/S0950268810001068. Epub 2010 May 18.
Recently, multiple-locus variable-number tandem-repeat analysis (MLVA) has been proposed as an alternative to pulsed-field gel electrophoresis (PFGE) for characterization of Escherichia coli O157:H7. In this study we characterized 118 E. coli O157:H7 isolates from cases of gastrointestinal disease in New Zealand using XbaI PFGE profiles and a MLVA scheme that assessed variability in eight polymorphic loci. The 118 isolates characterized included all 80 E. coli O157:H7 referred to New Zealand's Enteric Reference Laboratory in 2006 and 29 phage-type 2 isolates from 2005. When applied to these isolates the discriminatory power of PFGE and MLVA was not significantly different. However, MLVA data may be more epidemiologically relevant as isolates from family clusters of disease had identical MLVA profiles, even when the XbaI PFGE profiles differed slightly. Furthermore, most isolates with indistinguishable XbaI PFGE profiles that did not appear to be epidemiologically related had distinct MLVA profiles.
最近，多位点可变数目串联重复分析（MLVA）已被提议作为脉冲场凝胶电泳（PFGE）的替代方法，用于大肠杆菌 O157:H7 的特征分析。在这项研究中，我们使用 XbaI PFGE 图谱和评估 8 个多态性位点变异性的 MLVA 方案，对来自新西兰胃肠道疾病病例的 118 株大肠杆菌 O157:H7 进行了特征分析。所鉴定的 118 株包括 2006 年新西兰肠道参考实验室送检的所有 80 株大肠杆菌 O157:H7 和 2005 年的 29 株噬菌体型 2 株。当应用于这些分离株时，PFGE 和 MLVA 的区分能力没有显著差异。然而，MLVA 数据可能更具有流行病学相关性，因为疾病家族群中的分离株具有相同的 MLVA 图谱，即使 XbaI PFGE 图谱略有不同。此外，大多数 XbaI PFGE 图谱无法区分且似乎没有流行病学相关性的分离株具有不同的 MLVA 图谱。
