Department of Obstetrics/Gynecology, University of Washington, Seattle, WA, USA.
Am J Obstet Gynecol. 2010 Jul;203(1):32.e1-8. doi: 10.1016/j.ajog.2010.03.037. Epub 2010 May 15.
We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor.
Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression.
Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection.
Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.
我们分析了阴道液蛋白质组，以鉴定早产孕妇羊水中感染的生物标志物。
采用多维液相色谱、串联质谱和无标记定量技术对早产孕妇的阴道液标本进行蛋白质组分析。酶联免疫吸附试验用于定量候选蛋白。使用逻辑回归获得的受试者工作特征曲线评估宫内感染（羊水细菌培养阳性和/或白细胞介素-6>2ng/ml）的分类准确性。
170 例受试者中，30 例（18%）发生宫内感染。阴道液蛋白质组分析显示 338 种独特蛋白质。无标记定量鉴定出 15 种在宫内感染中表达差异的蛋白质，包括急性期反应物、免疫调节剂、高丰度羊水蛋白和细胞外基质信号因子；这些发现通过酶联免疫吸附试验得到了证实。多分析物算法准确地对宫内感染进行分类。
阴道液蛋白质组分析鉴定出了能够区分宫内感染患者和无感染患者的蛋白质。
