Erlich H A, Gelfand D, Sninsky J J
Department of Human Genetics, Core Technology, Cetus Corporation, Emeryville, CA 94608.
Science. 1991 Jun 21;252(5013):1643-51. doi: 10.1126/science.2047872.
The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.
聚合酶链反应（PCR）极大地改变了分子研究的开展方式以及能够提出的问题类型。除了简化通常使用重组DNA技术进行的分子任务外，PCR还带来了一系列进展，从新型基因和病原体的鉴定到特定核苷酸序列的定量分析。PCR能够深入了解单细胞的复杂性以及物种的进化。本文综述了PCR在仪器、方法和应用方面的一些最新进展。
