Wang Qianhui, Wang Jiaming, Zheng Yan, Li Changjiang, Dou Xingtang, Cao Zhongzan, Gao Yue, Xue Bing, Han Di, Luan Xinhong
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161, PR China.
Liaoning Province Modern Agricultural Production Base Construction Engineering Center, Liaoyang, Liaoning 110033, PR China.
J Genet Eng Biotechnol. 2025 Sep;23(3):100517. doi: 10.1016/j.jgeb.2025.100517. Epub 2025 Jun 14.
In this study, microsatellite loci in geese were initially screened, after which a multiplex PCR system was constructed and the genetic diversity and genetic structure of 40 samples from each of the three Huoyan goose populations in Liaoning were analyzed using the STR typing test. The results showed that 12 microsatellite loci were screened, and one quadruple PCR system, two triple PCR systems and one double PCR system were constructed with consistent and reliable reproducibility. A total of 90 alleles were detected in 120 samples, the number of alleles(Na), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content(PIC) of the three populations ranged from 5.083 to 6.083, from 0.149 to 0.184, from 0.555 to 0.577 and from 0.503 to 0.512.respectively, the populations present an unbalanced state, and the genetic diversity of three populations was highly polymorphic and highly inbred. The results of Principal Coordinates Analysis (PCoA) and genetic structure analysis showed that the genetic backgrounds of the three populations of Huoyan geese were similar, with close affinities and frequent gene exchanges.
本研究首先对鹅的微卫星位点进行筛选，之后构建多重PCR体系，并利用STR分型检测对辽宁地区3个豁眼鹅群体各40个样本的遗传多样性和遗传结构进行分析。结果表明，共筛选出12个微卫星位点，构建了1个四重PCR体系、2个三重PCR体系和1个双重PCR体系，体系重复性一致且可靠。在120个样本中共检测到90个等位基因，3个群体的等位基因数（Na）、观察杂合度（Ho）、期望杂合度（He）和多态信息含量（PIC）分别为5.0836.083、0.1490.184、0.5550.577和0.5030.512，群体呈现不平衡状态，3个群体的遗传多样性均为高度多态且高度自交。主坐标分析（PCoA）和遗传结构分析结果显示，3个豁眼鹅群体的遗传背景相似，亲缘关系较近且基因交流频繁。
