Adams M D, Kelley J M, Gocayne J D, Dubnick M, Polymeropoulos M H, Xiao H, Merril C R, Wu A, Olde B, Moreno R F
Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
Science. 1991 Jun 21;252(5013):1651-6. doi: 10.1126/science.2047873.
Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.
对600多个随机选择的人类大脑互补DNA（cDNA）克隆进行了自动化部分DNA测序，以生成表达序列标签（EST）。EST在发现新的人类基因、绘制人类基因组图谱以及识别基因组序列中的编码区域方面都有应用。在生成的序列中，337个代表新基因，其中48个与其他生物体的基因有显著相似性，如酵母RNA聚合酶II亚基；果蝇驱动蛋白、Notch和分裂增强子；以及小鼠酪氨酸激酶受体。46个EST在通过聚合酶链反应扩增后被定位到染色体上。这种快速的cDNA表征方法将在几年内以完成全基因组测序成本的一小部分促进大多数人类基因的标记，提供新的遗传标记，并作为多种生物学研究领域的资源。
