Clark R A, Winn H J, Dvorak H F, Colvin R B
Departments of Medicine and Dermatology, National Jewish Hospital, University of Colorado Medical School, Denver, Colorado, U.S.A., Departments of Surgery and Pathology, Massachusetts General Hospital, and Department of Pathology, Beth Israel Hospital and Harvard School of Medicine, Boston, Massachusetts, U.S.A.
J Invest Dermatol. 1983 Jun;80(1 Suppl):26s-30s. doi: 10.1038/jid.1983.7.
Fibronectin and fibrinogen occur under the migrating epidermal tongue during reepithelialization of an excisional wound, and fibronectin increases in conjunction with capillary and fibroblast ingrowth during wound healing. Although we have previously shown that fibronectin is produced by proliferating blood vessels, the source of fibronectin associated with reepithelialization and fibroblast ingrowth has not been determined. In this report we demonstrate that subepidermal fibronectin derives mostly from plasma early in reepithelialization of an cxcisional wound and comes from both plasma and in situ production late in reepithelialization. This finding was established by extirpating 3 mm of skin from the center of a well-healed rat xenograph on the flanks of immunosuppressed mice, harvesting the open wound sites at 2, 4, 7, and 10 days after injury, and staining the specimens with reciprocal species-specific anti-fibronec-tin antibodies conjugated with fluorescein. In the first 4 days after wounding, newly forming rat epidermis migrated mainly over mouse fibronectin. In contrast, by 7 days after excision, the rat epidermis transits over a matrix containing both mouse and rat fibronectin, or rat fibronectin alone, indicating that a major component of the fibronectin is produced in situ. Although the biologic significance of these observations has not been fully elucidated, fibronectin may be part of a provisional matrix that functions to support, if not actively participate in, cell recruitment to sites of inflammation or wound healing.
在切除伤口再上皮化过程中，纤连蛋白和纤维蛋白原出现在迁移的表皮舌下方，并且在伤口愈合期间，纤连蛋白随着毛细血管和成纤维细胞的长入而增加。尽管我们之前已经表明纤连蛋白由增殖的血管产生，但与再上皮化和成纤维细胞长入相关的纤连蛋白来源尚未确定。在本报告中，我们证明在切除伤口再上皮化早期，表皮下纤连蛋白主要来源于血浆，而在再上皮化后期则来自血浆和原位产生。这一发现是通过从免疫抑制小鼠侧腹愈合良好的大鼠异种移植皮肤中心切除3毫米皮肤，在损伤后第2、4、7和10天收集开放伤口部位，并用与荧光素偶联的种属特异性抗纤连蛋白抗体对标本进行染色而确定的。在受伤后的前4天，新形成的大鼠表皮主要在小鼠纤连蛋白上迁移。相反，到切除后7天，大鼠表皮在含有小鼠和大鼠纤连蛋白或仅含大鼠纤连蛋白的基质上迁移，这表明纤连蛋白的主要成分是原位产生的。尽管这些观察结果的生物学意义尚未完全阐明，但纤连蛋白可能是临时基质的一部分，其作用是支持（如果不是积极参与）细胞募集到炎症或伤口愈合部位。
