Clark R A, Quinn J H, Winn H J, Lanigan J M, Dellepella P, Colvin R B
J Exp Med. 1982 Aug 1;156(2):646-51. doi: 10.1084/jem.156.2.646.
During the time of tissue repair that ensues subsequent to tissue injury, blood vessel wall fibronectin increases concomitantly with endothelial proliferation and angiogenesis. However, the source of this blood vessel fibronectin had not been delineated. In this report we have demonstrated that microvascular fibronectin is produced in situ by the proliferating vessels surrounding excisional wounds. This finding was established by extirpating 3 mm of skin from the center of a well-healed rat xenograph on the flanks of immunosuppressed mice, harvesting the injured skin sites at various stages during the healing process, and staining the specimens with reciprocal species-specific anti-fibronectin. The proliferating donor vessels that surrounded the wounded graft had increased fluorescence staining with FITC conjugated mouse anti-rat fibronectin and no staining with rat anti-mouse fibronectin. This finding was taken as direct evidence that the fibronectin was produced in situ by the rat vessels and not derived from circulating mouse plasma.
在组织损伤后的组织修复期间，血管壁纤连蛋白随着内皮细胞增殖和血管生成而同步增加。然而，这种血管纤连蛋白的来源尚未明确。在本报告中，我们证明了微血管纤连蛋白是由切除伤口周围增殖的血管原位产生的。这一发现是通过从免疫抑制小鼠侧腹愈合良好的大鼠异种移植皮肤中心切除3毫米皮肤，在愈合过程的不同阶段采集受伤皮肤部位，并用种属特异性抗纤连蛋白抗体对标本进行染色而确定的。围绕受伤移植物的增殖供体血管在用异硫氰酸荧光素（FITC）偶联的小鼠抗大鼠纤连蛋白染色时荧光染色增加，而用大鼠抗小鼠纤连蛋白染色时无染色。这一发现被视为纤连蛋白是由大鼠血管原位产生而非来自循环小鼠血浆的直接证据。
