Seltzer J L, Horan T P, Eisen A Z
Division of Dermatology, Washington University School of Medicine, St. Louis, Missouri, U.S.A.
J Invest Dermatol. 1983 Jun;80(1 Suppl):42s-3s. doi: 10.1038/jid.1983.11.
Hydrocortisone and dexamethasone prevent the appearance of gelatinase in serum-free explant cultures of normal human skin. Hydrocortisone inhibits maximally at 10(-6) M and dexamethasone at 10(-8) M in culture medium. Glucocorticoids at these concentrations do not cause a generalized decrease in protein synthesis; thus the effect on gelatinase shows specificity. The reduction in gelatinase activity caused by dexamethasone can be overcome in the presence of dexamethasone 21-mesylate, a glucocorticoid antagonist that binds irreversibly to the cytoplasmic steroid receptor. These data suggest that the enzymes of collagen degradation, collagenase and gelatinase, may be coregulated.
氢化可的松和地塞米松可防止正常人皮肤无血清外植体培养物中明胶酶的出现。在培养基中，氢化可的松在10^(-6) M时抑制作用最大，地塞米松在10^(-8) M时抑制作用最大。这些浓度的糖皮质激素不会导致蛋白质合成普遍下降；因此，对明胶酶的作用具有特异性。地塞米松引起的明胶酶活性降低可在地塞米松21-甲磺酸盐存在下被克服，地塞米松21-甲磺酸盐是一种糖皮质激素拮抗剂，可不可逆地与细胞质类固醇受体结合。这些数据表明，胶原蛋白降解酶、胶原酶和明胶酶可能受到共同调节。
