Oikarinen A, Oikarinen H, Tan E M, Uitto J
Acta Derm Venereol. 1987;67(2):106-15.
Connective tissue metabolism was studied in detail in three human skin fibroblast lines, demonstrating low, medium, or high levels of glucocorticoid receptor densities. In the cell lines with low and medium receptor density, dexamethasone, in the range of 10(-5)-10(-9) M, had no effect on collagen production, using short incubation time periods and high (20%) fetal calf serum concentration, while in the cells with highest receptor density, a slight stimulation of collagen synthesis was noted in the concentration range 10(-6)-10(-9) M. In the presence of low concentration (0.5%) of serum, dexamethasone markedly inhibited collagen production. The production of collagenase, assayed by degradation of 3H-labelled type I collagen substrate with a brief trypsin activation of the enzyme, was reduced in a dose dependent manner in all 3 cell lines, the inhibition with 10(-5) dexamethasone being up to 56% of the control. Similarly, the activity of an elastase-like neutral protease was decreased in the presence of dexamethasone. Thus the results indicate that glucocorticoids may have profound effects on the degradation of connective tissue components, while the effects on collagen synthesis may be more variable depending on the environmental milieu.
在三种人皮肤成纤维细胞系中对结缔组织代谢进行了详细研究，这些细胞系分别显示出低、中、高糖皮质激素受体密度水平。在受体密度低和中等的细胞系中，使用短孵育时间和高（20%）胎牛血清浓度，10(-5)-10(-9)M范围内的地塞米松对胶原蛋白生成没有影响，而在受体密度最高的细胞中，在10(-6)-10(-9)M浓度范围内观察到胶原蛋白合成有轻微刺激。在低浓度（0.5%）血清存在下，地塞米松显著抑制胶原蛋白生成。通过用胰蛋白酶短暂激活酶来降解3H标记的I型胶原蛋白底物测定的胶原酶生成，在所有三种细胞系中均呈剂量依赖性降低，10(-5)地塞米松的抑制作用高达对照的56%。同样，在地塞米松存在下，弹性蛋白酶样中性蛋白酶的活性降低。因此，结果表明糖皮质激素可能对结缔组织成分的降解有深远影响，而对胶原蛋白合成的影响可能因环境背景而更具变化性。
