Seltzer J L, Horan T P, Eisen A Z
J Invest Dermatol. 1983 Jun;80 Suppl:42s-43s.
Hydrocortisone and dexamethasone prevent the appearance of gelatinase in serum-free explant cultures of normal human skin. Hydrocortisone inhibits maximally at 10(-6) M and dexamethasone at 10(-8) M in culture medium. Glucocorticoids at these concentrations do not cause a generalized decrease in protein synthesis; thus the effect on gelatinase shows specificity. The reduction in gelatinase activity caused by dexamethasone can be overcome in the presence of dexamethasone 21-mesylate, a glucocorticoid antagonist that binds irreversibly to the cytoplasmic steroid receptor. These data suggest that the enzymes of collagen degradation, collagenase and gelatinase, may be coregulated.
氢化可的松和地塞米松可防止正常人皮肤无血清外植体培养物中明胶酶的出现。氢化可的松在培养基中浓度为10(-6)M时抑制作用最强，地塞米松在浓度为10(-8)M时抑制作用最强。这些浓度的糖皮质激素不会导致蛋白质合成普遍减少；因此，对明胶酶的作用具有特异性。地塞米松21-甲磺酸盐是一种与细胞质类固醇受体不可逆结合的糖皮质激素拮抗剂，在其存在的情况下，地塞米松引起的明胶酶活性降低可被克服。这些数据表明，胶原蛋白降解酶，即胶原酶和明胶酶，可能受到共同调节。
