Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.
J Am Chem Soc. 2010 Jun 16;132(23):7838-9. doi: 10.1021/ja101677r.
We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.
我们提出了一种生物正交且模块化的偶联方法,用于通过[1,3]-偶极环加成反应高效地将有机染料和生物分子偶联到量子点(QDs)上。该方法使用了非配位性官能团,并结合了环加成反应的快速速率,从而实现了高效的偶联。我们已经将该方法应用于原位靶向具有[1,3]-偶极环加成反应活性的 norbornene 涂层量子点,以标记带有四嗪修饰蛋白的活癌细胞。