宫颈阴道分泌物和精液会抑制阴道乳杆菌产生的过氧化氢的杀菌活性。
Cervicovaginal fluid and semen block the microbicidal activity of hydrogen peroxide produced by vaginal lactobacilli.
机构信息
Mucosal Protection Laboratory, Thomas C, Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
出版信息
BMC Infect Dis. 2010 May 19;10:120. doi: 10.1186/1471-2334-10-120.
BACKGROUND
H2O2 produced by vaginal lactobacilli is believed to protect against infection, and H2O2-producing lactobacilli inactivate pathogens in vitro in protein-free salt solution. However, cervicovaginal fluid (CVF) and semen have significant H2O2-blocking activity.
METHODS
We measured the H2O2 concentration of CVF and the H2O2-blocking activity of CVF and semen using fluorescence and in vitro bacterial-exposure experiments.
RESULTS
The mean H2O2 measured in fully aerobic CVF was 23 +/- 5 microM; however, 50 microM H2O2 in salt solution showed no in vitro inactivation of HSV-2, Neisseria gonorrhoeae, Hemophilus ducreyii, or any of six BV-associated bacteria. CVF reduced 1 mM added H2O2 to an undetectable level, while semen reduced 10 mM added H2O2 to undetectable. Moreover, the addition of just 1% CVF supernatant abolished in vitro pathogen-inactivation by H2O2-producing lactobacilli.
CONCLUSIONS
Given the H2O2-blocking activity of CVF and semen, it is implausible that H2O2-production by vaginal lactobacilli is a significant mechanism of protection in vivo.
背景
阴道乳杆菌产生的 H2O2 被认为可以预防感染，并且在无蛋白盐溶液中，产 H2O2 的乳杆菌可以使病原体失活。然而，宫颈阴道液（CVF）和精液具有显著的 H2O2 阻断活性。
方法
我们使用荧光和体外细菌暴露实验测量了 CVF 的 H2O2 浓度和 CVF 及精液的 H2O2 阻断活性。
结果
完全需氧的 CVF 中的平均 H2O2 测量值为 23±5μM；然而，盐溶液中 50μM 的 H2O2 并未使 HSV-2、淋病奈瑟菌、杜克雷嗜血杆菌或六种 BV 相关细菌中的任何一种失活。CVF 将 1mM 加入的 H2O2 降低至无法检测的水平，而精液将 10mM 加入的 H2O2 降低至无法检测的水平。此外，只需添加 1%的 CVF 上清液即可消除产 H2O2 的乳杆菌对病原体的体外失活作用。
结论
鉴于 CVF 和精液的 H2O2 阻断活性，阴道乳杆菌产生的 H2O2 不太可能是体内保护的重要机制。
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