Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Biosens Bioelectron. 2010 Jul 15;25(11):2522-9. doi: 10.1016/j.bios.2010.04.015. Epub 2010 Apr 21.
An adenine-templated molecularly imprinted polymer (MIP) film, deposited on a poly(bithiophene) barrier film, served as the recognition element of a piezomicrogravimetric (acoustic) chemosensor. A 10MHz AT-cut shear-thickness-mode bulk-acoustic-wave quartz crystal resonator with Pt film electrodes was used as the signal transducer. Adenine electrooxidation was prevented by the barrier film. The MIP film was deposited by electrochemical co-polymerization of two functional monomers of bis(bithiophene) derivatives, bearing either the 18-crown-6 or dioxaborinane substituent, in the presence of the adenine template. A strong base solution was then used to extract the template. Completeness of the template removal was substantiated by the UV-vis, XPS, DPV, and EIS measurements. The chemosensor performance was evaluated with the piezoelectric microgravimetry detection at QCM under FIA conditions using a carrier acetonitrile-water (1:1, v:v) mixed solvent solution. The linear dynamic concentration range extended from at least 0.1 to 1mM for the 35 microL/min flow rate, and 100 microL volume of the injected adenine solution. The chemosensor selectivity allowed for discrimination of the adenine analyte from structurally and functionally related interferants, such as 2-aminopurine, guanine, and ascorbic acid. The determined from the FIA kinetic studies stability constant of the MIP-adenine complex, (18+/-2.4)x10(4)M(-1), was much higher than that of the MIP-(2-aminopurine), (650+/-90)M(-1), MIP-guanine, (122+/-11)M(-1), and MIP-(ascorbic acid), (92+/-10)M(-1), complexes. The concentration limit of detection was as low as 5 nM adenine for the 35 microL/min flow rate, and 1 mL volume of the injected sample solution.
基于腺嘌呤模板的分子印迹聚合物（MIP）薄膜沉积在聚（联噻吩）阻挡膜上，作为压电力（声学）化学传感器的识别元件。使用带有 Pt 薄膜电极的 10MHz AT 切剪切厚度模式体声波石英晶体谐振器作为信号转换器。阻挡膜防止腺嘌呤电氧化。MIP 薄膜通过在腺嘌呤模板存在下电化学共聚两种双噻吩衍生物的功能单体来沉积，这两种功能单体分别带有 18-冠-6 或二氧杂硼烷取代基。然后使用强碱溶液提取模板。通过 UV-vis、XPS、DPV 和 EIS 测量证实了模板去除的完整性。在 FIA 条件下，使用载有乙腈-水（1:1，v:v）混合溶剂溶液的 QCM 通过压电微重力检测评估了化学传感器的性能。对于 35μL/min 的流速和 100μL 注入腺嘌呤溶液的体积，该化学传感器的线性动态浓度范围至少扩展到 0.1 至 1mM。该化学传感器的选择性允许区分腺嘌呤分析物与结构和功能相关的干扰物，如 2-氨基嘌呤、鸟嘌呤和抗坏血酸。从 FIA 动力学研究中确定的 MIP-腺嘌呤配合物的稳定常数，（18+/-2.4）x10(4)M(-1)，远高于 MIP-（2-氨基嘌呤），（650+/-90）M(-1)，MIP-鸟嘌呤，（122+/-11）M(-1)，和 MIP-（抗坏血酸），（92+/-10）M(-1)，配合物。对于 35μL/min 的流速和 1mL 体积的注入样品溶液，浓度检测限低至 5nM 腺嘌呤。
