Bioorganic & Biophysical Chemistry Laboratory, Centre for Biomaterials Chemistry, Linnæus University, Kalmar SE-391 82, Sweden.
J Nanobiotechnology. 2014 Mar 21;12:8. doi: 10.1186/1477-3155-12-8.
The interaction between biotin and avidin is utilized in a wide range of assay and diagnostic systems. A robust material capable of binding biotin should offer scope in the development of reusable assay materials and biosensor recognition elements.
Biotin-selective thin (3-5 nm) films have been fabricated on hexadecanethiol self assembled monolayer (SAM) coated Au/quartz resonators. The films were prepared based upon a molecular imprinting strategy where N,N'-methylenebisacrylamide and 2-acrylamido-2-methylpropanesulfonic acid were copolymerized and grafted to the SAM-coated surface in the presence of biotin methyl ester using photoinitiation with physisorbed benzophenone. The biotinyl moiety selectivity of the resonators efficiently differentiated biotinylated peptidic or carbohydrate structures from their native counterparts.
Molecularly imprinted ultra thin films can be used for the selective recognition of biotinylated structures in a quartz crystal microbalance sensing platform. These films are stable for periods of at least a month. This strategy should prove of interest for use in other sensing and assay systems.
生物素与亲和素的相互作用广泛应用于各种分析和诊断系统。一种能够与生物素结合的坚固材料有望为可重复使用的分析材料和生物传感器识别元件的开发提供新的机会。
在十六硫醇自组装单层（SAM）涂覆的金/石英谐振器上制备了具有生物素选择性的薄（3-5nm）膜。这些薄膜是基于分子印迹策略制备的，在存在生物素甲酯的情况下，使用物理吸附的苯甲酮进行光引发，将 N,N'-亚甲基双丙烯酰胺和 2-丙烯酰胺基-2-甲基丙磺酸共聚并接枝到 SAM 涂覆的表面上。谐振器的生物素部分选择性能够有效地将生物素化的肽或碳水化合物结构与其天然对应物区分开来。
分子印迹超薄膜可用于在石英晶体微天平传感平台上对生物素化结构进行选择性识别。这些薄膜在至少一个月的时间内保持稳定。该策略有望在其他传感和分析系统中得到应用。
