Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Anal Chem. 2009 Dec 15;81(24):10061-70. doi: 10.1021/ac9020352.
A melamine piezomicrogravimetric (acoustic) chemosensor using a molecularly imprinted polymer (MIP) film has been devised and tested. The MIP films were prepared by electropolymerization of the melamine complexed by the functional monomer of the bis(bithiophene) derivative bearing an 18-crown-6 substituent 4. The structure of the MIP-melamine complex was visualized by the DFT B3LYP/3-21G(*) energy optimization calculations. The sensitivity and selectivity of the MIP film was improved by cross-linking the polymer with the bithianaphthene monomer 5 and the presence of the porogenic ionic liquid in the prepolymerization solution. After electropolymerization, the melamine template was extracted from the MIP film with an aqueous strong base solution. The measurements of UV-vis spectroscopy, X-ray photoelectron spectroscopy (XPS), DPV, and EIS as well as scanning electrochemical microscopy (SECM) imaging confirmed extraction of the melamine template from the MIP film and then rebinding of the melamine analyte while the film relative roughness and porosity was determined by atomic force microscopy (AFM) and scanning electron microscopy (SEM) imaging, respectively. The analytical as well as kinetic and thermodynamic parameters of the chemosensing were assessed under flow-injection analysis (FIA) conditions with piezoelectric microgravimetry (PM) detection. The linear concentration range for melamine detection was 5 nM to at least 1 mM with a limit of detection of approximately 5 nM. The chemosensor successfully discriminated the cyanuric acid, cyromazine, and ammeline interfering agents.
一种使用分子印迹聚合物（MIP）薄膜的三聚氰胺压电力（声学）化学传感器已经被设计和测试。MIP 薄膜是通过电聚合三聚氰胺与带有 18-冠-6 取代基的双噻吩衍生物的功能单体形成的复合物来制备的。MIP-三聚氰胺配合物的结构通过 DFT B3LYP/3-21G(*)能量优化计算来可视化。通过将聚合物与双噻吩并萘单体 5 交联并在预聚合溶液中存在致孔离子液体，可以提高 MIP 薄膜的灵敏度和选择性。电聚合后，用强碱性水溶液从 MIP 薄膜中提取三聚氰胺模板。紫外可见光谱、X 射线光电子能谱（XPS）、DPV 和 EIS 以及扫描电化学显微镜（SECM）成像的测量结果证实了 MIP 薄膜中三聚氰胺模板的提取，然后在薄膜相对粗糙度和孔隙率分别通过原子力显微镜（AFM）和扫描电子显微镜（SEM）成像确定时，三聚氰胺分析物的再结合。分析以及化学传感的动力学和热力学参数在流动注射分析（FIA）条件下进行评估，采用压电微重力（PM）检测。三聚氰胺检测的线性浓度范围为 5 nM 至至少 1 mM，检测限约为 5 nM。该化学传感器成功地区分了三聚氰胺的干扰物氰尿酸、环丙嗪和氨纶。
