Sminia T, van der Ende M B
Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Acta Histochem. 1991;90(1):43-50. doi: 10.1016/S0065-1281(11)80156-X.
Macrophages' subsets including dendritic cells were characterized in situ and in cell suspensions of Peyer's patches, intestinal villi, and mesenteric lymph nodes using monoclonal antibodies, lectins, and enzyme-histochemistry. By combined labelling procedures, it was found that Peyer's patches comprise dendritic cells in addition to 2 types of macrophages; dendritic cells were not observed among the various macrophage-like cells in the lamina propria of the villi. The lectins Dolichos biflorus and Griffonia simplicifolia I agglutinin appeared to be useful for discriminating between several subtypes of macrophages.
利用单克隆抗体、凝集素和酶组织化学技术，对派尔集合淋巴结、肠绒毛和肠系膜淋巴结的原位及细胞悬液中的巨噬细胞亚群（包括树突状细胞）进行了表征。通过联合标记程序发现，派尔集合淋巴结除了包含2种巨噬细胞外，还含有树突状细胞；在绒毛固有层的各种巨噬细胞样细胞中未观察到树突状细胞。凝集素双花扁豆凝集素和西非豆凝集素似乎有助于区分巨噬细胞的几种亚型。
