van Rees E P, Dijkstra C D, van der Ende M B, Janse E M, Sminia T
Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Immunology. 1988 Jan;63(1):79-85.
The ontogenetic development of macrophage subpopulations and Ia-positive non-lymphoid cells was studied in gut-associated tissue in fetal and neonatal Wistar rats. A two-step immunoperoxidase method was carried out on cryostat sections, a panel of monoclonal antibodies being applied and aimed specifically at rat macrophages (ED1, ED2 and ED3) and at Ia antigen (Ox4). The first ED1-positive macrophages appeared in the liver on Day 15 (gestational age), and they did not express Ia. In developing mesenteric lymph nodes and in the gut wall, macrophages were found for the first time on Day 17 and 18, respectively, of gestation. These early macrophages were also ED1-positive. Until birth, ED2 recognized few cells in the gut-associated tissue; on the day of birth this subpopulation showed a sudden and considerable increase. The distribution pattern of ED3-positive macrophages appeared to be the same in fetal and in adult rats; it was confined to lymph nodes and Peyer's patches. Ia-positive non-lymphoid cells appeared in the abdomen early in ontogeny. The first Ia-positive cells displayed dendritic features and were found on Day 15 of fetal life in the mesenchymal tissue between intestinal loops. A few days later, many Ia-positive cells with a dendritic appearance were demonstrable in the gut wall and in developing mesenteric lymph nodes. Their number increased rapidly during the following days. Based on these results, the existence of two differentiation lines for dendritic cells and classical macrophages is discussed. The function of early Ia-positive cells in the abdomen is suggested as not being an antigen-presenting one.
在新生和胎儿期的Wistar大鼠的肠道相关组织中，研究了巨噬细胞亚群和Ia阳性非淋巴细胞的个体发育。在低温恒温器切片上采用两步免疫过氧化物酶法，应用一组单克隆抗体，这些抗体特异性针对大鼠巨噬细胞（ED1、ED2和ED3）和Ia抗原（Ox4）。第一批ED1阳性巨噬细胞在妊娠第15天出现在肝脏中，且不表达Ia。在发育中的肠系膜淋巴结和肠壁中，巨噬细胞分别在妊娠第17天和18天首次被发现。这些早期巨噬细胞也是ED1阳性。直到出生，ED2在肠道相关组织中识别的细胞很少；在出生当天，这个亚群突然大幅增加。ED3阳性巨噬细胞的分布模式在胎儿和成年大鼠中似乎相同；它局限于淋巴结和派尔集合淋巴结。Ia阳性非淋巴细胞在个体发育早期出现在腹部。第一批Ia阳性细胞呈现树突状特征，在胎儿期第15天在肠袢之间的间充质组织中被发现。几天后，在肠壁和发育中的肠系膜淋巴结中可显示出许多具有树突状外观的Ia阳性细胞。在接下来的几天里，它们的数量迅速增加。基于这些结果，讨论了树突状细胞和经典巨噬细胞两条分化路线的存在。腹部早期Ia阳性细胞的功能被认为不是抗原呈递功能。
