Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
Int Arch Allergy Immunol. 2010;153(3):303-14. doi: 10.1159/000314372. Epub 2010 May 20.
The precise roles of T helper (Th)1-type and Th2-type cytokine responses in nickel (Ni)-induced allergic contact dermatitis have not yet been clearly defined. We investigated the involvement of Th2 cytokines in Ni-induced contact hypersensitivity reaction using GATA-3 transgenic (Tg) mice.
A Ni-titanium (Ti) alloy was implanted under the skin of GATA-3 Tg mice. A Ni solution was then injected 1 month after sensitization. The ear swelling response was measured at several time points after the injection; the cytokine levels in the skin were measured at 48 h after injection, and the serum levels of IgE were measured 1 month after injection. In addition, purified CD4+ splenic cells obtained from the GATA-3 Tg mice sensitized with the Ni-Ti alloy were infused into Rag-2(-/-) mice, and the ear swelling response of these mice after a further challenge with Ni solution was also measured.
Marked ear swelling and elevated serum IgE levels and skin tissue levels of IL-4 were observed in Ni-Ti-sensitized GATA-3 Tg mice. The Rag-2(-/-) mice transfused with the CD4+ splenic cells from the Ni-Ti alloy sensitized GATA-3 Tg mice showed a significantly more pronounced ear swelling response than the control mice.
We confirmed the participation of Th2-type immune reactions in Ni-induced allergy using GATA-3 Tg mice.
Th1 型和 Th2 型细胞因子反应在镍（Ni）诱导的过敏性接触性皮炎中的精确作用尚未明确。我们使用 GATA-3 转基因（Tg）小鼠研究了 Th2 细胞因子在 Ni 诱导的接触超敏反应中的作用。
将 Ni-钛（Ti）合金植入 GATA-3 Tg 小鼠的皮肤下。致敏 1 个月后，注入 Ni 溶液。在注射后几个时间点测量耳肿胀反应；注射后 48 小时测量皮肤中的细胞因子水平，并在注射后 1 个月测量血清 IgE 水平。此外，将从用 Ni-Ti 合金致敏的 GATA-3 Tg 小鼠中获得的纯化 CD4+脾细胞注入 Rag-2(-/-)小鼠，并测量这些小鼠在进一步用 Ni 溶液挑战后的耳肿胀反应。
在 Ni-Ti 致敏的 GATA-3 Tg 小鼠中观察到明显的耳肿胀和升高的血清 IgE 水平以及皮肤组织中 IL-4 的水平。输注了来自 Ni-Ti 合金致敏的 GATA-3 Tg 小鼠的 CD4+脾细胞的 Rag-2(-/-)小鼠表现出比对照小鼠更明显的耳肿胀反应。
我们使用 GATA-3 Tg 小鼠证实了 Th2 型免疫反应在 Ni 诱导的过敏中的参与。
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