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使用多孔银膜增强单分子检测

Enhanced Single-Molecule Detection using Porous Silver Membrane.

作者信息

Fu Yi, Lakowicz Joseph R

机构信息

Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, MD 21221, .

出版信息

J Phys Chem C Nanomater Interfaces. 2010 Apr 29;114(16):7492-7495. doi: 10.1021/jp911407c.

DOI:10.1021/jp911407c
PMID:20485474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2871712/
Abstract

We evaluated a commercial porous silver membrane as a support substrate for direct localization and visualization of single molecule events. We characterized the fluorescence behaviors of fluorescently labeled DNA oligonucleotides on the silver membranes. The fluorescence from the fluorescent probes that were immobilized on the porous silver is greatly enhanced. Additionally, correlated to reflectance contour image, it appears that enhanced fluorescence came from location close to the "valley" of the pore channels (or in the voids). These results are of great interest to increase the effectiveness of fluorescence-based single molecule DNA analysis.

摘要

我们评估了一种商用多孔银膜作为支持底物，用于单分子事件的直接定位和可视化。我们表征了荧光标记的DNA寡核苷酸在银膜上的荧光行为。固定在多孔银上的荧光探针发出的荧光大大增强。此外，与反射轮廓图像相关，增强的荧光似乎来自靠近孔道“谷”（或空隙）的位置。这些结果对于提高基于荧光的单分子DNA分析的有效性具有重要意义。

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