Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, Hannover, Germany.
Tissue Eng Part A. 2010 Oct;16(10):3043-53. doi: 10.1089/ten.TEA.2010.0131.
Polymeric materials used in biomedical devices, bioartificial organs, or for the fabrication of tissue engineering scaffolds should completely prevent the activation of the coagulation system and subsequent clot formation. Surface endothelialization is considered an important tool to optimize the blood compatibility of synthetic materials, as a functional endothelial cell layer on an artificial material may help control hemostasis and, therefore, provide a solution to improve the biocompatibility of these materials. Here we report on the endothelialization of poly 4-methyl-1-pentene (PMP) gas exchange membranes using human cord blood-derived late outgrowth endothelial colony forming cells. We achieved complete endothelialization of PMP membranes; and when seeded and cultivated on the membrane, cord blood-derived late outgrowth endothelial colony forming cells maintained both endothelial characteristics and functionality. Endothelialization resulted in significantly lower platelet adhesion and activation compared with unseeded membranes. Of importance, the endothelial layer had no major impact on gas permeability of PMP membranes. This study is a first promising step toward the development of a biofunctionalized surface for the use in gas exchange devices with blood contacting surfaces and a straightforward approach toward a long-term bio-hybrid lung replacement system.
用于生物医学设备、生物人工器官或组织工程支架制造的聚合材料应完全防止凝血系统的激活和随后的血栓形成。表面内皮化被认为是优化合成材料血液相容性的重要工具，因为人工材料上的功能性内皮细胞层有助于控制止血，从而为提高这些材料的生物相容性提供了一种解决方案。在这里，我们报告了使用人脐血衍生的晚期出芽内皮集落形成细胞对聚 4-甲基-1-戊烯（PMP）气体交换膜进行内皮化。我们实现了 PMP 膜的完全内皮化；并且当在膜上接种和培养时，脐血衍生的晚期出芽内皮集落形成细胞保持了内皮特征和功能。与未接种的膜相比，内皮化导致血小板黏附和激活显著降低。重要的是，内皮层对 PMP 膜的气体透过率没有重大影响。这项研究是朝着开发用于与血液接触的气体交换装置的生物功能化表面迈出的有希望的第一步，也是实现长期生物杂交肺替代系统的简单方法。
