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Amplification immunoassay for the determination of hepatitis B surface antigen.

作者信息

Seki A, Tamiya E, Karube I

机构信息

Research Center for Advanced Science and Technology, University of Tokyo, Japan.

出版信息

Appl Biochem Biotechnol. 1991 Mar;27(3):259-65. doi: 10.1007/BF02921540.

DOI:10.1007/BF02921540
PMID:2048904
Abstract

A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipopolysaccharide (LPS) was conjugated to anti-HBs antibody. Anti-HBs antibody was adsorbed to polystyrene beads. First, HBs were reacted to solid phase anti-HBs antibody (a-HBs). After the reaction, the beads were rinsed, and were then reacted with a-HBs-LPS. Then, LPS activity specifically bound to the beads was measured. HBs could be measured in the range of 10(-10)-10(-12) g/mL.

摘要

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本文引用的文献

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Drug sensor: liposome immunosensor for theophylline.
Anal Biochem. 1981 Dec;118(2):286-93. doi: 10.1016/0003-2697(81)90584-4.
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Chemiluminescence enzyme immunoassay of 17 alpha-hydroxyprogesterone using glucose oxidase and bis(2,4,6-trichlorophenyl) oxalate - fluorescent dye system.
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Clin Chim Acta. 1985 May 30;148(2):119-24. doi: 10.1016/0009-8981(85)90221-9.
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Development of a sensitive enzyme immunoassay for human epidermal growth factor (urogastrone).
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Enzyme amplification for immunoassays. Detection limit of one hundredth of an attomole.用于免疫测定的酶扩增。检测限为百亿分之一阿托摩尔。
J Immunol Methods. 1986 Feb 27;87(1):7-11. doi: 10.1016/0022-1759(86)90337-6.
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Comparison of colorimetric, fluorescent, and enzymatic amplification substrate systems in an enzyme immunoassay for detection of DNA-RNA hybrids.用于检测DNA-RNA杂交体的酶免疫测定中比色、荧光和酶促扩增底物系统的比较。
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