Wang H, Huang H H, Ding J, Wang Y H
College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China.
Water Sci Technol. 2010;61(11):2733-9. doi: 10.2166/wst.2010.053.
Sperm cell and embryo toxicity tests using the sea urchin Strongylocentrotus intermedius (S. intermedius) were performed to assess the toxicity of indoxacarb, a new widely used insecticide. New toxicity data for indoxacarb expressed as median effective concentration (EC(50)) were reported for the sea urchin species. When sperms and cells were exposed to the pesticide before fertilization, no significant inhibition in the fertilization success of S. intermedius (up to 40 mg/L) was observed. Developmental toxicity of the pesticide displayed a significant dose-related increase of larval malformations and differentiation arrest at concentrations of 0.1 mg/L to 40.0 mg/L at each cleavage, including the 2-cell stage, 4-cell, blastula, gastrula, prism and 4-arm pluteus stages. It seems that 4-arm pluteus is the most sensitive to indoxacarb with the EC(50) of 3.73 mg/L, two times less than that of the first cleavage stage. All these results indicate that more attentions should be paid to the potential marine pollutions caused by this pesticide indoxacarb.
使用中间球海胆（Strongylocentrotus intermedius）进行了精子细胞和胚胎毒性试验，以评估新型广泛使用的杀虫剂茚虫威的毒性。报告了茚虫威对中间球海胆物种的以半数有效浓度（EC(50)）表示的新毒性数据。当精子和细胞在受精前接触该农药时，未观察到中间球海胆的受精成功率有显著抑制（高达40 mg/L）。该农药的发育毒性在每次分裂（包括2细胞期、4细胞期、囊胚期、原肠胚期、棱柱期和四腕幼虫期）浓度为0.1 mg/L至40.0 mg/L时，显示出幼虫畸形和分化停滞的显著剂量相关增加。似乎四腕幼虫对茚虫威最敏感，EC(50)为3.73 mg/L，比第一次分裂期低两倍。所有这些结果表明，应更加关注茚虫威这种农药造成的潜在海洋污染。
