Wankhede S B, Prakash A, Kumari B, Chitlange S S
Department of Pharmaceutical Chemistry, Pad. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-401 018, India.
Indian J Pharm Sci. 2009 May;71(3):325-8. doi: 10.4103/0250-474X.56026.
Three simple, accurate and economical methods have been developed for the estimation of norfloxacin and ornidazole in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 273.0 nm (lambda(max) of norfloxacin) and 318.5 nm (lambda(max) of ornidazole), respectively, in 0.1N NaOH. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 297.0 nm (iso-absorptive point) and 318.5 nm (lambda(max) of ornidazole). Third method is first order derivative spectroscopy using 297.5 nm (zero cross for norfloxacin) and 264.0 nm (zero cross for ornidazole). The linearity was obtained in the concentration range of 4-20 mug/ml and 5-25 mug/ml for norfloxacin and ornidazole, respectively. The results of the analysis have been validated statistically and by recovery studies.
已开发出三种简单、准确且经济的方法用于测定片剂剂型中的诺氟沙星和奥硝唑。第一种方法基于联立方程，在0.1N氢氧化钠中，选择用于分析的波长分别为273.0nm（诺氟沙星的λmax）和318.5nm（奥硝唑的λmax）。第二种方法是Q分析方法，基于在两个选定波长297.0nm（等吸收点）和318.5nm（奥硝唑的λmax）处的吸光度比。第三种方法是一阶导数光谱法，使用297.5nm（诺氟沙星的零交叉点）和264.0nm（奥硝唑的零交叉点）。诺氟沙星和奥硝唑的线性范围分别为4 - 20μg/ml和5 - 25μg/ml。分析结果已通过统计学和回收率研究进行了验证。
